{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:07:05Z","timestamp":1774922825405,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Endodontic J"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to evaluate the antimicrobial activity of calcium hydroxide\u2010loaded poly(lactic\u2010co\u2010glycolic acid) nanoparticles (CH\u2010loaded PLGA NPs) on multi\u2010species biofilms. Human root blocks were prepared (<jats:italic>n<\/jats:italic>\u2009=\u200940), and multi\u2010species suspensions of <jats:italic>Candida albicans<\/jats:italic>, <jats:italic>Enterococcus faecalis<\/jats:italic> and <jats:italic>Streptococcus gordonii<\/jats:italic> were incubated within the root canals for 21\u2009days. Canals (<jats:italic>n<\/jats:italic>\u2009=\u200910\/group) were then medicated with saline solution (negative control), chlorhexidine (positive control), calcium hydroxide and CH\u2010loaded PLGA NPs for 7\u2009days. Samples taken from the 0.1\u2009mm root canal dentin were collected, and cell growth was detected by culture on BHI agar. The viable cell count of the Ca(OH)<jats:sub>2<\/jats:sub>, chlorhexidine gel and CH\u2010loaded PLGA NPs group was significantly lower than the normal saline group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). CH\u2010loaded PLGA NPs demonstrated a significant lower viable cell than Ca(OH)<jats:sub>2<\/jats:sub> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001); it has potential as a medicament for endodontic therapy.<\/jats:p>","DOI":"10.1111\/aej.12812","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T09:00:32Z","timestamp":1699606832000},"page":"89-96","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Efficacy of calcium hydroxide\u2010loaded poly(lactic\u2010co\u2010glycolic acid) biodegradable nanoparticles as an intracanal medicament against endodontopathogenic microorganisms in a multi\u2010species biofilm model"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4384-6404","authenticated-orcid":false,"given":"Warat","family":"Leelapornpisid","sequence":"first","affiliation":[{"name":"Department of Restorative Dentistry and Periodontology, Faculty of Dentistry Chiang Mai University  Chiang Mai Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piyaporn","family":"Wanwatanakul","sequence":"additional","affiliation":[{"name":"Maehia Dental Studio  Chiang Mai Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thanisorn","family":"Mahatnirunkul","sequence":"additional","affiliation":[{"name":"National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA)  Pathum Thani Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2001.00366.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(12)70901-5"},{"key":"e_1_2_8_4_1","article-title":"Novel PLGA\u2010based nanoparticles for the oral delivery of insulin","volume":"10","author":"Malathi S","year":"2015","journal-title":"Int J Nanomedicine"},{"key":"e_1_2_8_5_1","first-page":"219","volume-title":"Doxorubicin\u2010loaded PLGA nanoparticles by nanoprecipitation: preparation, characterization and in\u00a0vitro evaluation","author":"Betancourt T","year":"2007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2008.321"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5584268"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13050670"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2005.12.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25163731"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14071519"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10024-3522"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13603"},{"issue":"1","key":"e_1_2_8_14_1","article-title":"Antimicrobial activity of nanoparticle calcium hydroxide against enterococcus faecalis: an in\u00a0vitro study","volume":"10","author":"Dianat O","year":"2015","journal-title":"Iran Endod J"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2022.105539"},{"issue":"8","key":"e_1_2_8_16_1","first-page":"34","article-title":"Effectiveness of Maprang \u201cBouea macrophylla Griffith\u201d seed extract against endodontopathogenic microorganisms in a multispecies bacterial\u2010fungal biofilm","volume":"42","author":"Leelapornpisid W","year":"2021","journal-title":"Chiang Mai Dent J"},{"issue":"3","key":"e_1_2_8_17_1","first-page":"366","article-title":"Comparison of the penetration depth of conventional and nano\u2010particle calcium hydroxide into dentinal tubules","volume":"12","author":"Zand V","year":"2017","journal-title":"Iran Endod J"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.05.126"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.04.015"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2003.00634.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijos.2013.12"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1988.tb00949.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)80461-9"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2000.00291.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.49.4.359-378.1985"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2002.00504.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.1999.00195.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2003.12.022"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22122092"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11092438"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-012-0938-8"}],"container-title":["Australian Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aej.12812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T07:53:10Z","timestamp":1712389990000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aej.12812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,10]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/aej.12812"],"URL":"https:\/\/doi.org\/10.1111\/aej.12812","archive":["Portico"],"relation":{},"ISSN":["1329-1947","1747-4477"],"issn-type":[{"value":"1329-1947","type":"print"},{"value":"1747-4477","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,10]]},"assertion":[{"value":"2022-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}