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The instruments were divided into groups (<jats:italic>n<\/jats:italic>\u2009=\u20098): WOG and RM\u2014control groups, new instruments; WOG1 and RM1; WOG2 and RM2; WOG3 and RM3 after instrumentation of 1, 2 or 3 molars, respectively. Burrs occurred mainly in the control group and after the first use. The RM files were found to have a higher nickel content, which increased during reuse, and a decrease in oxygen content with increasing reuse, in addition to calcium impregnation, which occurred in greater amounts in the corrosion areas in the WOG files. The presence of topographic and chemical changes was demonstrated, indicating that caution should be taken when reusing endodontic instruments to avoid fractures.<\/jats:p>","DOI":"10.1111\/aej.12861","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T06:29:18Z","timestamp":1716359358000},"page":"502-512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In vitro study of structural and chemical changes in two reciprocating files after multiple reuses and sterilisation"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7866-5461","authenticated-orcid":false,"given":"Amanda Zambaldi","family":"Marim","sequence":"first","affiliation":[{"name":"Department of Endodontics, S\u00e3o Leopoldo Mandic School S\u00e3o Leopoldo Mandic Research Center  S\u00e3o Paulo 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