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Apical papilla tissues of immature extracted tooth roots were digested, trypsinized and resuspended as a single cell suspension stained with CD146, CD73 and CD90 antibodies. Results revealed that apical papilla tissue harbours hAP\u2010MSCs, and they can be cultured and characterised. Low\u2010concentration intracanal medicaments (0.025\u2009mg\/mL) were less cytotoxic compared to higher concentrations (0.1\u2009mg\/mL and above). Calcium hydroxide was better in terms of viability as well as osteogenic differentiation (at 0.025, 0.05 and 0.1\u2009mg\/mL concentration) than triple antibiotic paste (TAP), modified TAP, and DAP (double antibiotic paste) at 24, 48, and 72\u2009h. Thus, calcium hydroxide was less cytotoxic compared to the different antibiotic paste combinations.<\/jats:p>","DOI":"10.1111\/aej.70033","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T05:34:35Z","timestamp":1762839275000},"page":"110-123","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Intracanal Medicaments on Cell Cycle, Viability and Differentiation of Human Apical Papilla Mesenchymal Stem Cells (\n                    <scp>hAP<\/scp>\n                    \u2010\n                    <scp>MSCs<\/scp>\n                    )"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2924-8857","authenticated-orcid":false,"given":"Morankar","family":"Rahul","sequence":"first","affiliation":[{"name":"Division of Pedodontics and Preventive Dentistry, Centre for Dental Education and Research AIIMS  New Delhi 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