{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:56:43Z","timestamp":1785855403699,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Endodontic J"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This in\u00a0vitro study investigated the antibiofilm efficacy of calcium silicate\u2010based, Simvastatin, Levofloxacin and calcium hydroxide intracanal medicaments against mature\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    biofilm. Ninety dentine specimens were inoculated with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    biofilm for three weeks and divided into five groups (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200918): (1) calcium silicate\u2010based; (2) Simvastatin; (3) Levofloxacin; (4) calcium hydroxide (CH); (5) positive control, no medicament. After two weeks of intracanal medicament application, live\/dead bacterial cells were assessed using confocal laser scanning microscopy. The results revealed a statistically significant difference in the percentage of dead bacteria of the four tested groups compared to the positive control group (H\u2009=\u200930.45,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). The calcium silicate\u2010based group recorded the highest median (IQR) percentage of dead bacteria at 56.33 (10.03), followed by Simvastatin 44.88 (7.30), CH 41.70 (12.53), Levofloxacin 35.99 (3.78), and the positive control group 3.61 (1.01). These findings suggest that these intracanal medicaments demonstrate promising antibiofilm activity, with further investigations needed.\n                  <\/jats:p>","DOI":"10.1111\/aej.70070","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:51:33Z","timestamp":1772967093000},"page":"461-469","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Calcium Silicate\u2010Based, Simvastatin, Levofloxacin and Calcium Hydroxide Intracanal Medicaments Against Mature\n                    <scp>\n                      <i>Enterococcus faecalis<\/i>\n                    <\/scp>\n                    Biofilm. An In\u00a0Vitro Study"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8142-8628","authenticated-orcid":false,"given":"Nadia Saeed","family":"Hosny","sequence":"first","affiliation":[{"name":"Department of Endodontics, Faculty of Dentistry Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Gamal","family":"Elgamal","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Faculty of Dentistry Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2006.01099.x"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.07.028"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13731"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-024-00280-5"},{"key":"e_1_2_14_6_1","first-page":"63","article-title":"Effect of Vehicle on Antimicrobial Properties of Calcium Hydroxide Pastes","volume":"10","author":"Estrela C.","year":"1999","journal-title":"Brazilian Dental Journal"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2011.01886.x"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.08.017"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13070637"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12542"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12175"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2001.tb00172.x"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500608"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/aihb.aihb_141_23"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.53730\/ijhs.v9n1.15498"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200363240-00008"},{"issue":"1","key":"e_1_2_14_17_1","first-page":"40","article-title":"Combined Effect of Levofloxacin and N\u2010Acetylcysteine Against Enterococcus Faecalis Biofilm for Regenerative Endodontics: An in Vitro Study","volume":"14","author":"Khosravi M. R.","year":"2019","journal-title":"Iranian Endodontic Journal"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-77130-1"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb15060139"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13542"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/jphi.JPHI_26_17"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.34172\/joddd.2022.026"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.21608\/asdj.2023.244877.1192"},{"key":"e_1_2_14_24_1","first-page":"1090","article-title":"An Overview of Uses and Effectiveness of Intracanal Medicaments in Endodontics","volume":"12","author":"Alshargawi W. K.","year":"2025","journal-title":"International Journal of Community Medicine and Public Health"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102443"},{"key":"e_1_2_14_26_1","first-page":"992","article-title":"Confocal laser scanning microscopy","volume":"27","author":"Paddock S. W.","year":"1999","journal-title":"Biotechnology Techniques"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-016-0015-x"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14247594"},{"issue":"10","key":"e_1_2_14_29_1","article-title":"Evaluation of the Anti\u2010Microbial Efficacy of a Novel Endodontic Irrigant Against Enterococcus Faecalis: An in Vitro Study","volume":"15","author":"Selvakumar G.","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma17225441"}],"container-title":["Australian Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aej.70070","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aej.70070","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aej.70070","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:37:47Z","timestamp":1785854267000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aej.70070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,4]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/aej.70070"],"URL":"https:\/\/doi.org\/10.1111\/aej.70070","archive":["Portico"],"relation":{},"ISSN":["1329-1947","1747-4477"],"issn-type":[{"value":"1329-1947","type":"print"},{"value":"1747-4477","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,4]]},"assertion":[{"value":"2025-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}