{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:56:49Z","timestamp":1785855409836,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Endodontic J"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to establish a standardised protocol for isolating dental pulp stem cells (DPSCs) from fully impacted third molar teeth and to assess the effect of long\u2010term cryopreservation on their viability for future clinical applications in regenerative endodontics. DPSCs were isolated from 21 impacted third molars. The clinical relevance of these cells was characterised via flow cytometry, confirming that 96% expressed mesenchymal stem cell markers. Seventeen samples were cryopreserved at \u221286\u00b0C for up to 12\u2009months to evaluate biological stability for autologous transplantation. The standardised surgical extraction and isolation protocol yielded a high concentration of therapeutic\u2010grade cells (3.43\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    cells\/mL). Post\u2010thaw viability remained stable (74% to 99%) over 12\u2009months. Importantly, the cells maintained consistent morphology, suggesting they retained the biological potential necessary for dentine\u2010pulp complex regeneration. The results validate a reliable long\u2010term DPSC preservation protocol, supporting feasible dental stem cell banking and a source of viable cells for regenerative endodontic procedures.\n                  <\/jats:p>","DOI":"10.1111\/aej.70071","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:30:51Z","timestamp":1773405051000},"page":"470-479","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Potential of Long\u2010Term Cryopreserved Stem Cells From Impacted Third Molar Pulps for Regenerative Endodontic Procedures: An Ex\u00a0Vivo Evaluation"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7327-1010","authenticated-orcid":false,"given":"G\u00f6zde","family":"Kandemir Demirci","sequence":"first","affiliation":[{"name":"Department of Endodontics, Faculty of Dentistry Ege University  \u0130zmir T\u00fcrkiye"},{"name":"Department of Stem Cell, Institute of Health Science Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zg\u00fcn","family":"Germiyan","sequence":"additional","affiliation":[{"name":"Department of Stem Cell, Institute of Health Science Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birant","family":"\u015eim\u015fek","sequence":"additional","affiliation":[{"name":"Department of Stem Cell, Institute of Health Science Ege University  \u0130zmir T\u00fcrkiye"},{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dentistry Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Emin","family":"Kaval","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Faculty of Dentistry Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pelin","family":"G\u00fcneri","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Radiology, Faculty of Dentistry Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U\u011fur","family":"Tekin","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dentistry Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timur","family":"K\u00f6se","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Medical Informatics, Faculty of Medicine Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4016-0522","authenticated-orcid":false,"given":"Yi\u011fit","family":"Uyan\u0131kgil","sequence":"additional","affiliation":[{"name":"Department of Stem Cell, Institute of Health Science Ege University  \u0130zmir T\u00fcrkiye"},{"name":"Department of Histology and Embryology, Faculty of Medicine Ege University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511405327"},{"key":"e_1_2_13_3_1","first-page":"303","article-title":"Endodontic Regeneration: Hard Shell, Soft Core","volume":"109","author":"Widbiller M.","year":"2021","journal-title":"Molecules"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470167526.ch8"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0937635100"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.11.021"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2004.12.004"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509333804"},{"key":"e_1_2_13_10_1","first-page":"860","article-title":"Stem Cells\u2013Prospects in Dentistry","volume":"120","author":"Ulmer F. 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