{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:00:43Z","timestamp":1790398843984,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Endodontic J"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Oxidative stress in inflamed pulp tissue compromises dental pulp stem cell survival, yet whether bioceramic materials preserve mitochondrial homeostasis under such conditions remains unclear. The present study demonstrates that iRoot BP Plus, a premixed calcium silicate cement, protects human dental pulp stem cells (hDPSCs) from H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced oxidative injury through comprehensive mitochondrial protection. iRoot BP Plus significantly restored cell viability, reduced intracellular ROS accumulation, and stabilized mitochondrial membrane potential. Morphometric analysis revealed preserved mitochondrial network integrity with maintained aspect ratio and branching complexity. Mechanistically, iRoot BP Plus upregulated biogenesis regulators (PGC\u20101\u03b1, TFAM, NRF\u20101), suppressed fission gene DRP1 overexpression, and restored fusion gene MFN2 expression. These findings establish that iRoot BP Plus confers cytoprotection by maintaining mitochondrial homeostasis across structural, functional and transcriptional dimensions, providing mechanistic rationale for its clinical efficacy and highlighting mitochondrial protection as a therapeutic target in vital pulp therapy.\n                  <\/jats:p>","DOI":"10.1111\/aej.70126","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:39:48Z","timestamp":1790397588000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>iRoot BP<\/scp>\n                    Plus Sustains Mitochondrial Homeostasis in Human Dental Pulp Stem Cells Subjected to Oxidative Stress"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3372-0727","authenticated-orcid":false,"given":"Jiahe","family":"Li","sequence":"first","affiliation":[{"name":"Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"issue":"25","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"13625","DOI":"10.1073\/pnas.240309797","article-title":"Postnatal Human Dental Pulp Stem Cells (DPSCs) In\u00a0Vitro and In\u00a0Vivo","volume":"97","author":"Gronthos S.","year":"2000","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"9","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1177\/0022034509340867","article-title":"Mesenchymal Stem Cells Derived From Dental Tissues vs. Those From Other Sources: Their Biology and Role in Regenerative Medicine","volume":"88","author":"Huang G. T. J.","year":"2009","journal-title":"Journal of Dental Research"},{"issue":"2","key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.14744\/eej.2022.74745","article-title":"Observation of Inflammation, Oxidative Stress, Mitochondrial Dynamics, and Apoptosis in Dental Pulp Following a Diagnosis of Irreversible Pulpitis","volume":"8","author":"Vaseenon S.","year":"2023","journal-title":"European Endodontic Journal"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.redox.2015.01.002","article-title":"Oxidative Stress: A Concept in Redox Biology and Medicine","volume":"4","author":"Sies H.","year":"2015","journal-title":"Redox Biology"},{"issue":"1","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/BJ20081386","article-title":"How Mitochondria Produce Reactive Oxygen Species","volume":"417","author":"Murphy M. P.","year":"2009","journal-title":"Biochemical Journal"},{"issue":"5684","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1126\/science.1099320","article-title":"The Pathophysiology of Mitochondrial Cell Death","volume":"305","author":"Green D. R.","year":"2004","journal-title":"Science"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.01.021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5149"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102591.2003.00692.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100722.2003.00062.x"},{"issue":"1","key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/etp.12075","article-title":"Bioceramic Materials in Endodontics","volume":"32","author":"Wang Z.","year":"2015","journal-title":"Endodontic Topics"},{"issue":"1","key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s40368-018-0328-x","article-title":"Biodentine Material Characteristics and Clinical Applications: A 3 Year Literature Review and Update","volume":"19","author":"Rajasekharan S.","year":"2018","journal-title":"European Archives of Paediatric Dentistry"},{"issue":"11","key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/j.joen.2018.08.004","article-title":"Outcomes of Vital Pulp Therapy Using Mineral Trioxide Aggregate or Biodentine: A Prospective Randomized Clinical Trial","volume":"44","author":"Awawdeh L.","year":"2018","journal-title":"Journal of Endodontics"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2021.06.018"},{"issue":"11","key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1016\/j.joen.2011.07.006","article-title":"Role of SIRT1 in Heat Stress\u2010 and Lipopolysaccharide\u2010Induced Immune and Defense Gene Expression in Human Dental Pulp Cells","volume":"37","author":"Lee S. I.","year":"2011","journal-title":"Journal of Endodontics"},{"issue":"2","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s00784-015-1515-3","article-title":"Three\u2010Dimensional Culture of Dental Pulp Stem Cells in Direct Contact to Tricalcium Silicate Cements","volume":"20","author":"Widbiller M.","year":"2016","journal-title":"Clinical Oral Investigations"},{"issue":"1","key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1186\/s12903-022-02467-w","article-title":"Oxidative Stress Impairs the Calcification Ability of Human Dental Pulp Cells","volume":"22","author":"Shirawachi S.","year":"2022","journal-title":"BMC Oral Health"},{"key":"e_1_2_10_19_1","volume-title":"ISO 10993\u201012:2021. Biological Evaluation of Medical Devices. Part 12: Sample Preparation and Reference Materials","author":"International Organization for Standardization","year":"2021"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2017.03.001"},{"issue":"10","key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"R453","DOI":"10.1016\/j.cub.2014.03.034","article-title":"ROS Function in Redox Signaling and Oxidative Stress","volume":"24","author":"Schieber M.","year":"2014","journal-title":"Current Biology"},{"issue":"1","key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.joen.2009.09.006","article-title":"Mineral Trioxide Aggregate: A Comprehensive Literature Review\u2014Part I: Chemical, Physical, and Antibacterial Properties","volume":"36","author":"Parirokh M.","year":"2010","journal-title":"Journal of Endodontics"},{"issue":"6","key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1111\/j.1365-2591.2012.02013.x","article-title":"Chemical\u2013Physical Properties of TheraCal, a Novel Light\u2010Curable MTA\u2010Like Material for Pulp Capping","volume":"45","author":"Gandolfi M. G.","year":"2012","journal-title":"International Endodontic Journal"},{"issue":"17","key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1007\/s00018-016-2223-0","article-title":"Role of Nrf2\/HO\u20101 System in Development, Oxidative Stress Response and Diseases: An Evolutionarily Conserved Mechanism","volume":"73","author":"Loboda A.","year":"2016","journal-title":"Cellular and Molecular Life Sciences"},{"issue":"7","key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/j.jeurceramsoc.2008.08.025","article-title":"Ceramics for Medical Applications: A Picture for the Next 20\u2009Years","volume":"29","author":"Chevalier J.","year":"2009","journal-title":"Journal of the European Ceramic Society"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784\u2010021\u201003840\u20109"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb17010017"},{"issue":"4","key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.joen.2006.09.013","article-title":"Regenerative Endodontics: A Review of Current Status and a Call for Action","volume":"33","author":"Murray P. E.","year":"2007","journal-title":"Journal of Endodontics"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13778"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2026.02.017"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2147\/CIA.S158513","article-title":"Oxidative Stress, Aging, and Diseases","volume":"13","author":"Liguori I.","year":"2018","journal-title":"Clinical Interventions in Aging"},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ab.2017.07.009","article-title":"Mitochondrial Membrane Potential","volume":"552","author":"Zorova L. D.","year":"2018","journal-title":"Analytical Biochemistry"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1146\/annurev-genet-110410-132529","article-title":"Fusion and Fission: Interlinked Processes Critical for Mitochondrial Health","volume":"46","author":"Chan D. C.","year":"2012","journal-title":"Annual Review of Genetics"},{"issue":"6098","key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1126\/science.1219855","article-title":"Mitochondrial Fission, Fusion, and Stress","volume":"337","author":"Youle R. J.","year":"2012","journal-title":"Science"},{"issue":"7","key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1016\/j.bbamcr.2010.09.019","article-title":"Metabolic Control of Mitochondrial Biogenesis Through the PGC\u20101 Family Regulatory Network","volume":"1813","author":"Scarpulla R. C.","year":"2011","journal-title":"Biochimica et Biophysica Acta (BBA) \u2010 Molecular Cell Research"}],"container-title":["Australian Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aej.70126","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aej.70126","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aej.70126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:39:56Z","timestamp":1790397596000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aej.70126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":34,"alternative-id":["10.1111\/aej.70126"],"URL":"https:\/\/doi.org\/10.1111\/aej.70126","archive":["Portico"],"relation":{},"ISSN":["1329-1947","1747-4477"],"issn-type":[{"value":"1329-1947","type":"print"},{"value":"1747-4477","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"assertion":[{"value":"2026-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"aej.70126"}}