{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T05:40:52Z","timestamp":1696398052662},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T00:00:00Z","timestamp":1374451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Abnormal phenotypes in cloned pigs can be partly due to changes in epigenetic modifications such as methylation levels of promoter CpG islands. <jats:italic>Neuronatin<\/jats:italic> is an imprinted gene, conserved in human, pig, cattle and mouse, which is expressed exclusively from the paternal allele. Three CpG islands located in the promoter region of the porcine <jats:italic>neuronatin<\/jats:italic> gene have the potential to regulate the gene expression by cytosine methylation. To illustrate whether <jats:italic>neuronatin<\/jats:italic> was differentially expressed among nuclear transfer macroglossia\u2013positive and nuclear transfer macroglossia\u2013negative pigs and <jats:italic>in vitro\u2010<\/jats:italic>fertilized pigs, we detected its expression level by <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010PCR and further quantified methylation levels by pyrosequencing DNA from the liver. The results showed that <jats:italic>neuronatin<\/jats:italic> was expressed at a significantly higher level in livers of nuclear transfer macroglossia\u2010positive pigs compared with normal cloned and <jats:italic>in vitro<\/jats:italic>\u2010fertilized pigs. Livers of nuclear transfer macroglossia\u2010positive pigs also had a significantly lower methylation level at CpG island 2 and CpG island 3 in the promoter region.<\/jats:p>","DOI":"10.1111\/age.12074","type":"journal-article","created":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T09:08:42Z","timestamp":1374484122000},"page":"122-124","source":"Crossref","is-referenced-by-count":3,"title":["Methylation differences of the <i>neuronatin<\/i> gene promoter region in liver between normal and cloned pigs"],"prefix":"10.1111","volume":"45","author":[{"given":"Ting","family":"Gu","sequence":"first","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education &amp; Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Su","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education &amp; Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education &amp; Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changchun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education &amp; Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,22]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20786"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr419"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241522698"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025901"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043325"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.9.1543"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.051722"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.017293"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001849"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-1000-x"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fage.12074","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.12074","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T13:22:53Z","timestamp":1696339373000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/age.12074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,22]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/age.12074"],"URL":"https:\/\/doi.org\/10.1111\/age.12074","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,22]]}}}