{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T16:10:53Z","timestamp":1774714253320,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T00:00:00Z","timestamp":1396483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Growth hormone releasing hormone (<jats:styled-content style=\"fixed-case\">GHRH<\/jats:styled-content>) regulates the secretion of growth hormone (<jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content>) in the pituitary gland. A 66\u2010bp deletion (c.\u2010923_\u2010858del) was detected in the 5\u2032\u2010flanking sequence of the largemouth bass (<jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>icropterus salmoides<\/jats:italic>)<jats:italic><jats:styled-content style=\"fixed-case\">GHRH<\/jats:styled-content><\/jats:italic>gene. In two cultured random populations of adult individuals (A:<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>170 and B:<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>150), the genotype ratios of +\/+:+\/\u2212 were 2.5:1 and 2.8:1 respectively. Only one \u2212\/\u2212 fish was detected. A<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>argemouth bass family was constructed with two heterozygous individuals (+\/\u2212) as parents. The genotype ratio of +\/+:+\/\u2212:\u2212\/\u2212 in the filial generation embryos was 1:1.6:0.1 at the neurula and 1:2:0 at hatched larvae stages. This indicated that the 66\u2010bp deletion was a recessive lethal site and that homozygous individuals (\u2212\/\u2212) died off in embryonic development. The growth traits (body weight, body length and body depth) were measured, and the<jats:italic><jats:styled-content style=\"fixed-case\">GHRH<\/jats:styled-content><\/jats:italic>m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>expression levels in brain tissue were detected using real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The effects of genotype (+\/\u2212) on growth traits and<jats:italic><jats:styled-content style=\"fixed-case\">GHRH<\/jats:styled-content><\/jats:italic>m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>expression were not significant. Although the cause of death was not clear, the results hint that the 66\u2010bp deletion site in<jats:italic><jats:styled-content style=\"fixed-case\">GHRH<\/jats:styled-content><\/jats:italic>5\u2032\u2010flanking sequence significantly affects the livability in largemouth bass embryonic development.<\/jats:p>","DOI":"10.1111\/age.12143","type":"journal-article","created":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T05:54:37Z","timestamp":1396504477000},"page":"421-426","source":"Crossref","is-referenced-by-count":7,"title":["A 66\u2010bp deletion in<i>growth hormone releasing hormone<\/i>gene 5\u2032\u2010flanking region with largemouth bass recessive embryonic lethal"],"prefix":"10.1111","volume":"45","author":[{"given":"D. M.","family":"Ma","sequence":"first","affiliation":[{"name":"Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation Ministry of Agriculture of the People's Republic of China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Q.","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation Ministry of Agriculture of the People's Republic of China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J.","family":"Bai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation Ministry of Agriculture of the People's Republic of China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. J.","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation Ministry of Agriculture of the People's Republic of China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J.","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation Ministry of Agriculture of the People's Republic of China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Y.","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation Ministry of Agriculture of the People's Republic of China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. 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