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Live poultry, chicken eggs and meat are primary sources of human salmonellosis. To understand the genetic resistance of egg\u2010type chickens in response to <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation, global gene expression in the spleen of 20\u2010week\u2010old White Leghorn was measured using the Agilent 4\u00a0\u00d7\u00a044 K chicken microarray at 7 and 14\u00a0days following <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation (dpi). Results showed that there were 1363 genes significantly differentially expressed between inoculated and non\u2010inoculated groups at 7 dpi (I7\/N7), of which 682 were up\u2010regulated and 681 were down\u2010regulated genes. By contrast, 688 differentially expressed genes were observed at 14 dpi (I14\/N14), of which 371 were up\u2010regulated genes and 317 were down\u2010regulated genes. There were 33 and 28 immune\u2010related genes significantly differentially expressed in the comparisons of I7\/N7 and I14\/N14 respectively. Functional annotation revealed that several Gene Ontology (<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>) terms related to immunity were significantly enriched between the inoculated and non\u2010inoculated groups at 14 dpi but not at 7 dpi, despite a similar number of immune\u2010related genes identified between I7\/N7 and I14\/N14. The immune response to <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation changes with different time points following <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation. The complicated interaction between the immune system and metabolism contributes to the immune responses to <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation of egg\u2010type chickens at 14 dpi at the onset of lay. <jats:italic><jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> TNFSF<\/jats:styled-content>8, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>86, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>274, <jats:styled-content style=\"fixed-case\">BLB<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">BLB<\/jats:styled-content>2<\/jats:italic> play important roles in response to <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation. The results from this study will deepen the current understanding of the genetic response of the egg\u2010type chicken to <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content> inoculation at the onset of egg laying.<\/jats:p>","DOI":"10.1111\/age.12341","type":"journal-article","created":{"date-parts":[[2015,9,11]],"date-time":"2015-09-11T08:48:53Z","timestamp":1441961333000},"page":"617-626","source":"Crossref","is-referenced-by-count":20,"title":["Splenic gene expression profiling in White Leghorn layer inoculated with the <i><scp>S<\/scp>almonella enterica<\/i> serovar <scp>E<\/scp>nteritidis"],"prefix":"10.1111","volume":"46","author":[{"given":"Guixian","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Shandong Agricultural University Taian Shandong 271018 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liying","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Science 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