{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:14:37Z","timestamp":1790511277612,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,9,11]],"date-time":"2015-09-11T00:00:00Z","timestamp":1441929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministry of Education","award":["BES-2014-FPU13\/04975"],"award-info":[{"award-number":["BES-2014-FPU13\/04975"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A genomic region in pig chromosome 4 has been previously associated with higher viraemia levels and lower weight gain following porcine reproduction and respiratory syndrome virus (<jats:styled-content style=\"fixed-case\">PRRSV<\/jats:styled-content>) infection. The region includes the marker <jats:italic><jats:styled-content style=\"fixed-case\">WUR<\/jats:styled-content>1000125<\/jats:italic>, a G&gt;A polymorphism next to a putative polyadenylation site in the 3\u2019\u2010untranslated region (3\u2019\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content>) of the <jats:italic>guanylate\u2010binding protein 1, interferon\u2010induced<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content>1<\/jats:italic>) gene. The protein encoded by <jats:italic><jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content>1<\/jats:italic> is a negative regulator of T\u2010cell responses. We show here that <jats:italic><jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content>1<\/jats:italic> expression is lower in liver and tonsils of pigs carrying the <jats:italic><jats:styled-content style=\"fixed-case\">WUR<\/jats:styled-content>1000125\u2010G<\/jats:italic> allele due to differential allele expression (allele <jats:italic>A<\/jats:italic> expression is 1.9\u2010fold higher than for allele <jats:italic>G<\/jats:italic>). We also show that the <jats:italic><jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content>1<\/jats:italic> gene has two active polyadenylation signals 421\u00a0bp apart and that polyadenylation usage is dependent on the <jats:italic><jats:styled-content style=\"fixed-case\">WUR<\/jats:styled-content>1000125<\/jats:italic> genotype. The distal site is the most prevalently used in all samples, but the presence of the <jats:italic>A<\/jats:italic> allele favours the generation of shorter transcripts from the proximal site. This is confirmed by a differential allele expression study in <jats:italic><jats:styled-content style=\"fixed-case\">AG<\/jats:styled-content><\/jats:italic> genotype liver and tonsil samples. The interaction between <jats:italic><jats:styled-content style=\"fixed-case\">WUR<\/jats:styled-content>1000125<\/jats:italic> and other mutations identified in the 5\u2019\u2010 and 3\u2019\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> regions of this gene needs to be studied. In conclusion, our study indicates that the <jats:italic><jats:styled-content style=\"fixed-case\">WUR<\/jats:styled-content>1000125<\/jats:italic> mutation is associated with changes in the expression of the negative T\u2010cell regulator <jats:italic><jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content>1<\/jats:italic> gene. However, the chromosome 4 locus for <jats:styled-content style=\"fixed-case\">PRRSV<\/jats:styled-content> viraemia levels and weight gain contains a cluster of four other <jats:styled-content style=\"fixed-case\">GBP<\/jats:styled-content> genes that remain to be studied as candidate genes for this <jats:styled-content style=\"fixed-case\">QTL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/age.12347","type":"journal-article","created":{"date-parts":[[2015,9,11]],"date-time":"2015-09-11T09:29:28Z","timestamp":1441963768000},"page":"599-606","source":"Crossref","is-referenced-by-count":16,"title":["Expression profiling of the <i><scp>GBP<\/scp>1<\/i> gene as a candidate gene for porcine reproductive and respiratory syndrome resistance"],"prefix":"10.1111","volume":"46","author":[{"given":"S.","family":"Gol","sequence":"first","affiliation":[{"name":"Department of Animal Production University of Lleida\u2010Agrotecnio Center 25198 Lleida Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Estany","sequence":"additional","affiliation":[{"name":"Department of Animal Production University of Lleida\u2010Agrotecnio Center 25198 Lleida Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. J.","family":"Fraile","sequence":"additional","affiliation":[{"name":"Department of Animal Production University of Lleida\u2010Agrotecnio Center 25198 Lleida Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. N.","family":"Pena","sequence":"additional","affiliation":[{"name":"Department of Animal Production University of Lleida\u2010Agrotecnio Center 25198 Lleida Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,11]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"321","article-title":"Characterizing differential individual response to porcine reproductive and respiratory syndrome virus infection through statistical and functional analysis of gene expression","volume":"3","author":"Arceo M.E.","year":"2012","journal-title":"Frontiers in Genetics"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-0990-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2011-4464"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/v4050833"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg500"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300377"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl333"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2012.01.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1201711"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2008-0892"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1006603"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3210"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.05.022"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt997"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/18.2.333"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20031873"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00664-13"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-9-292"},{"key":"e_1_2_6_20_1","first-page":"583","volume-title":"XV National Animal Production Meeting","author":"Pena R.","year":"2013"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0388.2009.00818.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1857-8"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13253-013-0162-x"},{"key":"e_1_2_6_24_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"2001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003320"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-7821"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjs041"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm306"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-0992-y"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-1039-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011377"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-85"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055920"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fage.12347","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.12347","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T13:39:46Z","timestamp":1696513186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/age.12347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,11]]},"references-count":32,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/age.12347"],"URL":"https:\/\/doi.org\/10.1111\/age.12347","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,11]]}}}