{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T05:51:51Z","timestamp":1785736311592,"version":"3.56.0"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,10,18]],"date-time":"2015-10-18T00:00:00Z","timestamp":1445126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30972100"],"award-info":[{"award-number":["30972100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We previously reported that numerous naturally occurring genetic mutations in the 5\u2032\u2010upstream regulatory region (5\u2032\u2010<jats:styled-content style=\"fixed-case\">URR<\/jats:styled-content>) of the bovine <jats:italic>follicle\u2010stimulating hormone beta\u2010subunit<\/jats:italic> gene (<jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic>) were associated with reduced serum follicle\u2010stimulating hormone (<jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content>) levels, poor\u2010quality semen and low fertility in bulls. In addition, two different <jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> transcripts resulting from the linked mutations of genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> were discovered in mutation\u2010bearing bull pituitaries. Here, using electrophoretic mobility shift assay, we identified c.\u20101539_\u20101538del<jats:styled-content style=\"fixed-case\">GG<\/jats:styled-content>ins<jats:styled-content style=\"fixed-case\">TTAACT<\/jats:styled-content> mutations in the 5\u2032\u2010<jats:styled-content style=\"fixed-case\">URR<\/jats:styled-content> that generated a novel cis\u2010regulatory element in bovine <jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic>. Moreover, this novel element seemed to play a role in repressing <jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic> transcription based on a promoter activity analysis in L<jats:italic>\u03b2<\/jats:italic>T2 gonadotrope cells. Quantitative assays of <jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in the bovine pituitaries suggested that the levels of <jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content> wild\u2010type transcripts in the mutation\u2010bearing bulls were significantly lower (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) than in those of bulls without <jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic> genetic mutations and that the levels of <jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic>\u2010mutated transcripts were significantly lower (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) than that of wild\u2010type transcripts in the mutation\u2010bearing bulls. Altogether, our results suggest that decreased serum <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> levels and male fertility in bulls with the c.\u20101539_\u20101538del<jats:styled-content style=\"fixed-case\">GG<\/jats:styled-content>ins<jats:styled-content style=\"fixed-case\">TTAACT<\/jats:styled-content> mutation likely result from the alteration of cis\u2010regulatory elements and induction of <jats:italic><jats:styled-content style=\"fixed-case\">FSHB<\/jats:styled-content><\/jats:italic> transcription.<\/jats:p>","DOI":"10.1111\/age.12348","type":"journal-article","created":{"date-parts":[[2015,10,19]],"date-time":"2015-10-19T04:36:46Z","timestamp":1445229406000},"page":"693-696","source":"Crossref","is-referenced-by-count":4,"title":["Naturally occurring genetic mutations in the 5\u2032\u2010upstream regulatory region of bovine <i><scp>FSHB<\/scp><\/i> generate a novel cis\u2010regulatory element that affects its expression"],"prefix":"10.1111","volume":"46","author":[{"given":"Lisheng","family":"Dai","sequence":"first","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangyang","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianjiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tenghe","family":"Ma","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center College of Animal Sciences Jilin University Changchun 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