{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:20:29Z","timestamp":1785774029096,"version":"3.56.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Veterinary Genetics Diagnostic Laboratory (LDGV)"},{"name":"Molecular and Applied Animal Cytogenetic Laboratory (MERAGEM Research Group), University of C\u00f3rdoba"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Chromosomal abnormalities in the sex chromosome pair (<jats:styled-content style=\"fixed-case\">ECAX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ECAY<\/jats:styled-content>) are widely associated with reproductive problems in horses. However, a large proportion of these abnormalities remains undiagnosed due to the lack of an affordable diagnostic tool that allows for avoiding karyotyping tests. Hereby, we developed an <jats:styled-content style=\"fixed-case\">STR<\/jats:styled-content> (single\u2010tandem\u2010repeat)\u2010based molecular method to determine the presence of the main sex chromosomal abnormalities in horses in a fast, cheap and reliable way. The frequency of five <jats:styled-content style=\"fixed-case\">ECAX<\/jats:styled-content>\u2010linked <jats:italic>(<jats:styled-content style=\"fixed-case\">LEX<\/jats:styled-content>026, <jats:styled-content style=\"fixed-case\">LEX<\/jats:styled-content>003, <jats:styled-content style=\"fixed-case\">TKY<\/jats:styled-content>38, <jats:styled-content style=\"fixed-case\">TKY<\/jats:styled-content>270 and <jats:styled-content style=\"fixed-case\">UCDEQ<\/jats:styled-content>502)<\/jats:italic> and two <jats:styled-content style=\"fixed-case\">ECAY<\/jats:styled-content>\u2010linked (<jats:italic>Eca<jats:styled-content style=\"fixed-case\">YH<\/jats:styled-content>12 and <jats:styled-content style=\"fixed-case\">SRY<\/jats:styled-content><\/jats:italic>) markers was characterized in 261 Purebred Spanish Horses to determine the efficiency of the methodology developed to be used as a chromosomal diagnostic tool. All the microsatellites analyzed were highly polymorphic, with a sizeable number of alleles (polymorphic information content\u00a0&gt;\u00a00.5). Based on this variability, the methodology showed 100% sensitivity and 99.82% specificity to detect the most important sex chromosomal abnormalities reported in horses (chimerism, Turner's syndrome and sex reversal syndromes). The method was also validated with 100% efficiency in 10 individuals previously diagnosed as chromosomally aberrant. This <jats:styled-content style=\"fixed-case\">STR<\/jats:styled-content> screening panel is an efficient and reliable molecular\u2013cytogenetic tool for the early detection of sex chromosomal abnormalities in equines that could be included in breeding programs to save money, effort and time of veterinary practitioners and breeders.<\/jats:p>","DOI":"10.1111\/age.12543","type":"journal-article","created":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T06:42:48Z","timestamp":1487745768000},"page":"412-419","source":"Crossref","is-referenced-by-count":21,"title":["Sex chromosomal abnormalities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spanish Horse"],"prefix":"10.1111","volume":"48","author":[{"given":"G.","family":"Anaya","sequence":"first","affiliation":[{"name":"Laboratorio de Diagn\u00f3stico Gen\u00e9tico Veterinario Departamento de Gen\u00e9tica Universidad de C\u00f3rdoba CN IV KM 396 Edificio Gregor\u00a0Mendel Campus Rabanales C\u00f3rdoba 14071 Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Molina","sequence":"additional","affiliation":[{"name":"Laboratorio de Diagn\u00f3stico Gen\u00e9tico Veterinario Departamento de Gen\u00e9tica Universidad de C\u00f3rdoba CN IV KM 396 Edificio Gregor\u00a0Mendel Campus Rabanales C\u00f3rdoba 14071 Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Valera","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Agroforestales Escuela Tecnica Superior de Ingenier\u00eda Agron\u00f3mica Universidad de Sevilla Ctra de Utrera km 1 41013 Sevilla Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Moreno\u2010Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Citogen\u00e9tica Animal Aplicada\u00a0y\u00a0Molecular Departamento de Gen\u00e9tica Universidad de C\u00f3rdoba CN IV KM 396 Edificio Gregor Mendel Campus Rabanales C\u00f3rdoba 14071 Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Azor","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Agroforestales Escuela Tecnica Superior de Ingenier\u00eda Agron\u00f3mica Universidad de Sevilla Ctra de Utrera km 1 41013 Sevilla Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Peral\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Gen\u00e9tica Veterinaria \u201cIng. Fernando N. Dulout\u201d (UNLP \u2010 CONICET LA PLATA) Facultad de Ciencias\u00a0Veterinarias Universidad Nacional de La Plata Calle 60 y 118 s\/n 1900 La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3286-2441","authenticated-orcid":false,"given":"S.","family":"Demyda\u2010Peyr\u00e1s","sequence":"additional","affiliation":[{"name":"Instituto de Gen\u00e9tica Veterinaria \u201cIng. Fernando N. 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