{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T20:47:23Z","timestamp":1778186843236,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T00:00:00Z","timestamp":1494547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Knowledge Transfer Partnership"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Balanced chromosomal aberrations have been shown to affect fertility in most species studied, often leading to hypoprolificacy (reduced litter size) in domestic animals such as pigs. With an increasing emphasis in modern food production on the use of a small population of high quality males for artificial insemination, the potential economic and environmental costs of hypoprolific boars, bulls, rams etc. are considerable. There is therefore a need for novel tools to facilitate rapid, cost\u2010effective chromosome translocation screening. This has previously been achieved by standard karyotype analysis; however, this approach relies on a significant level of expertise and is limited in its ability to identify subtle, cryptic translocations. To address this problem, we developed a novel device and protocol for translocation screening using subtelomeric probes and fluorescence <jats:italic>in\u00a0situ<\/jats:italic> hybridisation. Probes were designed using <jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content>s (bacterial artificial chromosomes) from the subtelomeric region of the short (p\u2010arm) and long (q\u2010arm) of each porcine chromosome. They were directly labelled with <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content> or Texas Red (p\u2010arm and q\u2010arm respectively) prior to application of a \u2018Multiprobe\u2019 device, thereby enabling simultaneous detection of each individual porcine chromosome on a single slide. Initial experiments designed to isolate <jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content>s in subtelomeric regions led to the discovery of a series of incorrectly mapped regions in the porcine genome assembly (from a total of 82 <jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content>s, only 45 <jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content>s mapped correctly). Our work therefore highlights the importance of accurate physical mapping of newly sequenced genomes. The system herein described allows for robust and comprehensive analysis of the porcine karyotype, an adjunct to classical cytogenetics that provides a valuable tool to expedite efficient, cost effective food production.<\/jats:p>","DOI":"10.1111\/age.12548","type":"journal-article","created":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T10:09:28Z","timestamp":1494583768000},"page":"395-403","source":"Crossref","is-referenced-by-count":27,"title":["Isolation of subtelomeric sequences of porcine chromosomes for translocation screening reveals errors in the pig genome assembly"],"prefix":"10.1111","volume":"48","author":[{"given":"R. E.","family":"O'Connor","sequence":"first","affiliation":[{"name":"School of Biosciences University of Kent Canterbury CT2 7AF UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Fonseka","sequence":"additional","affiliation":[{"name":"Cytocell Ltd Newmarket Road Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Frodsham","sequence":"additional","affiliation":[{"name":"Cytocell Ltd Newmarket Road Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. L.","family":"Archibald","sequence":"additional","affiliation":[{"name":"The Roslin Institute R(D)SVS University of Edinburgh Division of Genetics and Genomics Easter Bush Midlothian EH25 9RG UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lawrie","sequence":"additional","affiliation":[{"name":"Cytocell Ltd Newmarket Road Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A.","family":"Walling","sequence":"additional","affiliation":[{"name":"JSR Genetics Southburn Driffield North Humberside YO25 9ED UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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