{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:43:41Z","timestamp":1764377021963,"version":"3.37.3"},"reference-count":8,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012818","name":"Community of Madrid","doi-asserted-by":"crossref","award":["IND2018\/BIO-9258"],"award-info":[{"award-number":["IND2018\/BIO-9258"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Spanish Ministry of Economy and Competitiveness","award":["Q2818014I"],"award-info":[{"award-number":["Q2818014I"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Four loci seem responsible for the dilution of the basic coat colours in horse: Dun (D), Silver Dapple (Z), Champagne (CH) and Cream (C). Apart from the current phenotypes ascribed to these loci, pearl has been described as yet another diluted coat colour in this species. To date, this coat colour seems to segregate only in the Iberian breeds Purebred Spanish horse and Lusitano and has also been described in breeds of Iberian origin, such as Quarter Horses and Paint Horse, where it is referred to as the \u2018Barlink Factor\u2019. This phenotype segregates in an autosomal recessive manner and resembles some of the coat colours produced by the champagne CH<jats:sup>CH<\/jats:sup> and cream C<jats:sup>Cr<\/jats:sup> alleles, sometimes being difficult to distinguish among them. The interaction between compound heterozygous for the pearl C<jats:sup>prl<\/jats:sup> and cream C<jats:sup>Cr<\/jats:sup> alleles makes <jats:italic>SLC45A2<\/jats:italic> the most plausible candidate gene for the pearl phenotype in horses. Our results provide documented evidence for the missense variation in exon 4 [<jats:italic>SLC45A2<\/jats:italic>:c.985G&gt;A; <jats:italic>SLC45A2<\/jats:italic>:p.(Ala329Thr)] as the causative mutation for the pearl coat colour. In addition, it is most likely involved as well in the cremello, perlino and smoky cream like phenotypes associated with the compound C<jats:sup>Cr<\/jats:sup> and C<jats:sup>prl<\/jats:sup> heterozygous genotypes (known as cream pearl in the Purebred Spanish horse breed). The characterization of the pearl mutation allows breeders to identify carriers of the C<jats:sup>prl<\/jats:sup> allele and to select this specific coat colour according to personal preferences, market demands or studbook requirements as well as to verify segregation within particular pedigrees.<\/jats:p>","DOI":"10.1111\/age.12784","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T11:38:36Z","timestamp":1554896316000},"page":"275-278","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Explicit evidence for a missense mutation in exon 4 of <i>SLC45A2<\/i> gene causing the pearl coat dilution in horses"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4766-6291","authenticated-orcid":false,"given":"N.","family":"Sevane","sequence":"first","affiliation":[{"name":"Departamento de Producci\u00f3n Animal Facultad de Veterinaria Universidad Complutense Madrid 28040 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3485-6785","authenticated-orcid":false,"given":"C. R.","family":"Sanz","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal Facultad de Veterinaria Universidad Complutense Madrid 28040 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8637-2208","authenticated-orcid":false,"given":"S.","family":"Dunner","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal Facultad de Veterinaria Universidad Complutense Madrid 28040 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,10]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.65.6.1375"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.12790"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9686-35-1-119"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.185"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0388.2009.00832.x"},{"volume-title":"Equine Color Genetics","year":"2009","author":"Sponenberg D.P.","key":"e_1_2_4_7_1"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(01)00218-2"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38548"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fage.12784","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.12784","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/age.12784","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.12784","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T11:29:01Z","timestamp":1694345341000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/age.12784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,10]]},"references-count":8,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/age.12784"],"URL":"https:\/\/doi.org\/10.1111\/age.12784","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"type":"print","value":"0268-9146"},{"type":"electronic","value":"1365-2052"}],"subject":[],"published":{"date-parts":[[2019,4,10]]},"assertion":[{"value":"2019-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}