{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:56:47Z","timestamp":1768456607445,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["3137231292"],"award-info":[{"award-number":["3137231292"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> (<jats:italic>imprinted gene in the Prader\u2010Willi syndrome region<\/jats:italic>), a long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, is a paternally expressed gene in the <jats:styled-content style=\"fixed-case\">PWS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content> imprinted domain on human chromosome 15 and mouse chromosome 7. Disruption of the <jats:styled-content style=\"fixed-case\">PWS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content> region is associated with three neurogenic disorders in humans. In this study, we identified the bovine homolog of the <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> gene; multiple transcripts obtained by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RACE<\/jats:styled-content> showed a complex and tissue\u2010specific expression pattern of <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> in the brain, heart, kidney, liver, lung, spleen and skeletal muscle. An informative single nucleotide polymorphism (rs133341090) in the long exon H was identified by sequencing the genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>, and mono\u2010allelic expression of <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> was confirmed by sequencing the <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content>s of heterozygous individuals, indicating that <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> may be imprinted in cattle. The protein\u2010coding potential of <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> transcripts was assessed using <jats:sc>coding potential calculator<\/jats:sc> (<jats:sc>cpc<\/jats:sc>) software, which showed a negative score. In addition, sequencing analysis also indicated multiple small open reading frames in the bovine <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> transcript, but none of the <jats:styled-content style=\"fixed-case\">ATG<\/jats:styled-content>s was consistent with Kozak consensus. Taken together, the <jats:italic><jats:styled-content style=\"fixed-case\">IPW<\/jats:styled-content><\/jats:italic> transcripts are most likely long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s.<\/jats:p>","DOI":"10.1111\/age.12815","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T09:19:22Z","timestamp":1562145562000},"page":"417-422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The molecular structure and imprinting status of the <i><scp>IPW<\/scp><\/i> (<i>imprinted gene in the Prader\u2010Willi syndrome region<\/i>) gene in cattle"],"prefix":"10.1111","volume":"50","author":[{"given":"W.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 China"},{"name":"College of Traditional Chinese Medicine Hebei University Baoding 071002 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ma","sequence":"additional","affiliation":[{"name":"Baoding NO.2 Hospital Baoding 071000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Life Science Hebei Agriculture University Baoding 071001 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