{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:33:05Z","timestamp":1758817985890,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T00:00:00Z","timestamp":1567382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802046"],"award-info":[{"award-number":["31802046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The yattle (dzo) is an interspecific hybrid of the taurine cattle (<jats:italic>Bos taurus<\/jats:italic>) and the domestic yak (<jats:italic>Bos grunniens<\/jats:italic>). F<jats:sub>1<\/jats:sub> hybrid yattle bulls are sterile due to spermatogenic arrest and have misregulation of spermatogenesis genes in the testes. However, the expression pattern of Y chromosome\u2010linked genes in cattle, yaks and yattle testes is still unknown. In this study, we analyzed the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression pattern of 10 genes known to be present as single copies in the X\u2010degenerate region of the bovine male\u2010specific region of the Y chromosome. Using male\u2010specific primers and reverse transcription quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, the <jats:italic>ubiquitously transcribed tetratricopeptide repeat gene, Y\u2010linked<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">UTY<\/jats:styled-content><\/jats:italic>), <jats:italic>oral\u2010facial\u2010digital syndrome 1, Y\u2010linked<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">OFD<\/jats:styled-content>1Y<\/jats:italic>) and <jats:italic>ubiquitin specific peptidase 9, Y\u2010linked<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content>9Y<\/jats:italic>) genes were ubiquitously expressed and significantly more highly expressed in yattle than in cattle and yaks testes (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001). <jats:italic><jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> binding motif protein, Y\u2010linked<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">RBMY<\/jats:styled-content><\/jats:italic>) had testes\u2010specific expression, and <jats:italic>eukaryotic translation initiation factor 1A, Y\u2010linked<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">EIF<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">AY<\/jats:styled-content><\/jats:italic>) was expressed mainly in testis, whereas yattle and cattle did not show significant differences with respect to the expression of <jats:italic><jats:styled-content style=\"fixed-case\">RBMY<\/jats:styled-content><\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">EIF<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">AY<\/jats:styled-content><\/jats:italic>. Thus, based on the model of yattle bull sterility, the high expression of <jats:italic><jats:styled-content style=\"fixed-case\">UTY<\/jats:styled-content><\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">OFD<\/jats:styled-content>1Y<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content>9Y<\/jats:italic> may be associated with yattle infertility.<\/jats:p>","DOI":"10.1111\/age.12841","type":"journal-article","created":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T08:35:12Z","timestamp":1567413312000},"page":"740-743","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of <scp>mRNA<\/scp> expression from Y\u2010chromosome X\u2010degenerate region genes in taurine cattle, yaks and interspecific hybrid bulls"],"prefix":"10.1111","volume":"50","author":[{"given":"Y.","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Science Southwest University Rongchang Chongqing 402460 China"},{"name":"Beef Cattle Engineering and Technology Research Center of Chongqing Southwest University Rongchang Chongqing 402460 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.\u2010X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Animal Husbandry and Veterinary Bureau of Rongchang Rongchang Chongqing 402460 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Zuo","sequence":"additional","affiliation":[{"name":"College of Animal Science Southwest University Rongchang Chongqing 402460 China"},{"name":"Beef Cattle Engineering and Technology Research Center of Chongqing Southwest University Rongchang Chongqing 402460 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9043-6945","authenticated-orcid":false,"given":"G.\u2010W.","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science Southwest University Rongchang Chongqing 402460 China"},{"name":"Beef Cattle Engineering and Technology Research Center of Chongqing Southwest University Rongchang Chongqing 402460 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,2]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.133967.111"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0817-2_9"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1169588"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.1.97"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.09.047"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026195"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1221104110"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.05.010"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2010.07.008"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12915"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03002"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.07.021"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12794"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/S1110724301000080"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-2030-z"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.555052"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-014-3611-x"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2016.04.049"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018-15680"}],"container-title":["Animal 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