{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T10:31:30Z","timestamp":1774780290145,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771372"],"award-info":[{"award-number":["31771372"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cloned animals are prone to abnormal phenotypes such as enlarged tongue, fetal oversize, and progeria. In the present study, whole\u2010genome bisulfite sequencing and mRNA sequencing were performed on tongue and biceps femoris muscles of cloned piglets with and without macroglossia, in an attempt to elucidate the epigenetic causes of the macroglossia phenotype. We identified 14\u00a0958 and 18\u00a0752 differentially methylated regions in the tongue and biceps femoris muscles, respectively, of macroglossia piglets and these correspond to 4574 and 4772 differentially methylated genes compared with the control group (piglets without macroglossia). Larger methylation difference was found in tongue muscle than in biceps femoris muscle. In total, 114 genes in tongue and 72 genes in biceps femoris muscles were found to be differentially expressed between the two groups. Of these differentially expressed genes in tongue muscle, 31 were also differentially methylated genes, among which <jats:italic>DIO3<\/jats:italic> and <jats:italic>ZIC1<\/jats:italic> were imprinting or predicted imprinting genes. These two and another six overlapping genes (<jats:italic>ALDH1A2<\/jats:italic>, <jats:italic>MKX<\/jats:italic>, <jats:italic>MAB21L2<\/jats:italic>, <jats:italic>CA3<\/jats:italic>, <jats:italic>RANBP3L<\/jats:italic>, and <jats:italic>MYL10<\/jats:italic>) are crucial factors involved in embryonic development or tissue and organ development. GO enrichment analysis suggested possible alteration of these processes. Our study provides novel molecular insights into the formation of macroglossia in cloned pigs.<\/jats:p>","DOI":"10.1111\/age.13105","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T21:50:47Z","timestamp":1624917047000},"page":"608-620","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome\u2010wide DNA methylation and transcription analysis in tongue and biceps femoris muscles of cloned pigs with macroglossia"],"prefix":"10.1111","volume":"52","author":[{"given":"Q.","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"Qiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. W.","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Y.","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q. Q.","family":"Jing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Y.","family":"Liao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6433-3221","authenticated-orcid":false,"given":"Y. Y.","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pig Genetic Improvement and Production Technology Jiangxi Agricultural University Nanchang 330045 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2015.1078050"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00121-15"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21308"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.22.010402.102745E"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2010.01899.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3032"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1198-z"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.02.006"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1360\/03yc0015"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2008.08.006"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0159-6"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.12074"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5505"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0950-z"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007203430305"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06546"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.163"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3230"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063852"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2017.12.004"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.02.026"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.05.011"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr167"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-811"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019428"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1237905"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00207-10"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.111.1.233"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw029"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1226"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.06.028"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21715"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.4.1001"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2018.05.003"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2003.1326"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063206"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032991"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032812"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-029X.140922"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000904"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/153623002320253283"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18040885"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.24.1.1566"},{"key":"e_1_2_10_48_1","article-title":"Detection of differentially methylated regions from whole\u2010genome bisulfite sequencing data without replicates","volume":"43","author":"Wu H.","year":"2015","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1203"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39883"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130275"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-2-r14"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45950"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-1000-x"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.12436"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.13105","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/age.13105","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.13105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T23:17:22Z","timestamp":1692832642000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/age.13105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,28]]},"references-count":54,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1111\/age.13105"],"URL":"https:\/\/doi.org\/10.1111\/age.13105","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,28]]},"assertion":[{"value":"2021-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}