{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T17:40:09Z","timestamp":1770226809665,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T00:00:00Z","timestamp":1639526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We developed a high\u2010resolution and comprehensive typing method for swine leukocyte antigen 3 (<jats:italic>SLA\u20103<\/jats:italic>), an MHC class I gene, employing locus\u2010specific genomic PCR followed by subsequent direct sequencing. A total of 292 individuals from nine pure, one cross\u2010breed and six cell lines were successfully typed. A total of 21 <jats:italic>SLA\u20103<\/jats:italic> alleles were identified, of which four were found to be novel alleles. However, the allelic diversity of <jats:italic>SLA\u20103<\/jats:italic> was lower than that of previously reported class I genes, <jats:italic>SLA\u20101<\/jats:italic> and \u2010<jats:italic>2<\/jats:italic>. More <jats:italic>SLA\u20103<\/jats:italic> alleles were observed in the Landrace and Yorkshire breeds than the other breeds. <jats:italic>SLA\u2010<\/jats:italic>3<jats:italic>*04:01<\/jats:italic> was identified in seven out of nine breeds and was the most widely distributed allele across all breeds. Therefore, the typing method reported in this study completes our efforts to develop high\u2010resolution typing methods for major SLA molecules, facilitating the combined analysis of major <jats:italic>SLA<\/jats:italic> genes from field samples, which is important to understand the relationship between the adaptive immune responses against pathogens and the immunogenetic makeup of an individual.<\/jats:p>","DOI":"10.1111\/age.13161","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T15:28:02Z","timestamp":1639582082000},"page":"166-170","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of a high\u2010resolution typing method for SLA\u20103, swine MHC class I antigen 3"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6970-4397","authenticated-orcid":false,"given":"S.","family":"Youk","sequence":"first","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.T.","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"},{"name":"Livestock Support Department National Agricultural Cooperative Federation Agribusiness Group Seoul 04516 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Animal Genomics and Bioinformatics Division National Institute of Animal Science RDA Wanju 55365 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1232-5307","authenticated-orcid":false,"given":"J.H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5520-2537","authenticated-orcid":false,"given":"C.S.","family":"Ho","sequence":"additional","affiliation":[{"name":"Gift of Hope Organ and Tissue Donor Network 425 Spring Lake Drive Itasca IL 60143 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4855-2210","authenticated-orcid":false,"given":"C.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biotechnology Konkuk University Gwangjin\u2010gu Seoul 05029 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-018-1073-3"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6165083"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12648"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.1994.tb00373.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.2009.00853.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.03.049"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12017"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-57712-5"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(91)90197-I"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.07.002"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.01.006"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(02)00015-6"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2010.01522.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-015-0883-9"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2008030"},{"key":"e_1_2_5_17_1","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.4049\/jimmunol.135.3.2167","article-title":"Structure and expression of two porcine genomic clones encoding class I MHC antigens","volume":"135","author":"Satz M.L.","year":"1985","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/36.3.306"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2011.01662.x"},{"key":"e_1_2_5_20_1","first-page":"1024","article-title":"Expression of a class I MHC transgene: regulation by a tissue\u2010specific negative regulatory DNA sequence element","volume":"2","author":"Frels W.I.","year":"1990","journal-title":"New Biologist"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-020-03624-0"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.13161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/age.13161","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.13161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T13:23:38Z","timestamp":1692969818000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/age.13161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,15]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1111\/age.13161"],"URL":"https:\/\/doi.org\/10.1111\/age.13161","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"value":"0268-9146","type":"print"},{"value":"1365-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,15]]},"assertion":[{"value":"2021-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}