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In our previous research, <jats:italic>SPAG6<\/jats:italic> c.900\u2009T&gt;C in exon 7 and exon 7 skipped transcript was identified by analyzing RNA\u2010seq data of testicular tissues from 60 day (sexually immature) and 180 day (sexually mature) Large White boars. Herein, we found porcine <jats:italic>SPAG6<\/jats:italic> c.900\u2009T&gt;C to be associated with semen quality traits in Duroc, Large White and Landrace pigs. <jats:italic>SPAG6<\/jats:italic> c.900 C can generate a new splice acceptor site, inhibit the occurrence of <jats:italic>SPAG6<\/jats:italic> exon 7 skipping to a certain extent, thereby promote the growth of Sertoli cells and maintain the normal blood\u2013testis barrier function. This study provides new insights into the molecular regulation of spermatogenesis and a new genetic marker for the improvement of semen quality in pigs.<\/jats:p>","DOI":"10.1111\/age.13330","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T04:50:28Z","timestamp":1684731028000},"page":"446-456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>SPAG6<\/i> c.900 T&gt;C affects boar semen quality and blood\u2013testis barrier function by creating a new splice acceptor site"],"prefix":"10.1111","volume":"54","author":[{"given":"Rong","family":"Bai","sequence":"first","affiliation":[{"name":"Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education Huazhong Agricultural University  Wuhan 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