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Here, we used low\u2010pass sequencing data with a median coverage of 0.64\u00d7 generated from blood samples of 322 F1 crosses between beef and dairy cattle and identified 20 putative blood chimeras through increased levels of genome\u2010wide heterozygosity. In contrast, for 77 samples with routine SNP microarray data generated from hair bulbs of the same F1s, we found no evidence of chimerism, simultaneously observing high levels of genotype discordance with sequencing data. Fifteen out of 18 reported twins showed signs of blood chimerism, in line with previous reports, whereas the presence of five alleged singletons with strong signs of chimerism suggests that the <jats:italic>in\u2010utero<\/jats:italic> death rate of co\u2010twins is at the upper limit of former estimates. Together, our results show that low\u2010pass sequencing data allow reliable screening for blood chimeras. They further affirm that blood is not recommended as a source of DNA for the detection of germline variants.<\/jats:p>","DOI":"10.1111\/age.13334","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T08:41:58Z","timestamp":1686732118000},"page":"613-618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increased heterozygosity in low\u2010pass sequencing data allows identification of blood chimeras in cattle"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1339-1410","authenticated-orcid":false,"given":"Dorothea","family":"Lindtke","sequence":"first","affiliation":[{"name":"Agroscope  Posieux Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz R.","family":"Seefried","sequence":"additional","affiliation":[{"name":"Qualitas AG  Zug Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9773-522X","authenticated-orcid":false,"given":"Cord","family":"Dr\u00f6gem\u00fcller","sequence":"additional","affiliation":[{"name":"Institute of Genetics University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Neuditschko","sequence":"additional","affiliation":[{"name":"Agroscope  Posieux Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.07.027"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/63.2.419"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","unstructured":"Buoen L.C. 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