{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:34:46Z","timestamp":1747182886733,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In mammals, imprinted genes are characterised by a monoallelic expression, which is based on parental origin and is essential for both foetal and placental development. The <jats:italic>ZFAT<\/jats:italic> gene encodes a transcriptional factor, and its non\u2010coding antisense RNA, <jats:italic>ZFAT\u2010AS1<\/jats:italic>, overlaps with the <jats:italic>ZFAT<\/jats:italic> locus. Both <jats:italic>ZFAT<\/jats:italic> and <jats:italic>ZFAT\u2010AS1<\/jats:italic> are maternally imprinted in human placentas. In bovines, the imprinting status of the <jats:italic>ZFAT<\/jats:italic> and <jats:italic>ZFAT\u2010AS1<\/jats:italic> genes has yet to be reported. In this study, we analysed the allelic expression of three transcript variants (X1\u2013X3) of the bovine <jats:italic>ZFAT<\/jats:italic> and <jats:italic>ZFAT\u2010AS1<\/jats:italic> genes in somatic tissues and placentas using a single nucleotide polymorphism\u2010based method. The results showed that bovine <jats:italic>ZFAT<\/jats:italic> exhibited isoform\u2010specific paternal expression. The <jats:italic>ZFAT<\/jats:italic> X2 variant exhibited monoallelic expression in the bovine placentas and biallelic expression in the six bovine somatic tissues (heart, liver, spleen, lung, kidney and brain). However, the <jats:italic>ZFAT<\/jats:italic> X1 and X3 variants were biallelically expressed in both bovine tissues and placentas. A 311\u2009bp bovine <jats:italic>ZFAT\u2010AS1<\/jats:italic> complementary DNA (cDNA) sequence was obtained by aligning the human <jats:italic>ZFAT\u2010AS1<\/jats:italic> cDNA sequence with the bovine genome and conducting reverse transcription polymerase chain reaction amplification. Bovine <jats:italic>ZFAT\u2010AS1<\/jats:italic> have monoallelic expression in bovine placentas and somatic tissues. In addition, the DNA methylation of two regions was characterised, including the partial promoter, and exon 1 and intron 1 regions of <jats:italic>ZFAT<\/jats:italic>, and there were no differentially methylated regions.<\/jats:p>","DOI":"10.1111\/age.13473","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T17:28:20Z","timestamp":1725470900000},"page":"820-824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>ZFAT<\/i> (isoform\u2010specific) and its antisense <scp>RNA<\/scp> 1 (<i>ZFAT\u2010AS1<\/i>) are two allele\u2010specific monoallelically expressed genes in cattle"],"prefix":"10.1111","volume":"55","author":[{"given":"Yinjiao","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Life Science Agricultural University of Hebei  Baoding Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunchang","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Science Agricultural University of Hebei  Baoding Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenli","family":"Yu","sequence":"additional","affiliation":[{"name":"Hebei Cattle Industry Technology Research Institute  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science Agricultural University of Hebei  Baoding Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science Agricultural University of Hebei  Baoding Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujing","family":"Li","sequence":"additional","affiliation":[{"name":"Hebei Provincial Dairy Cow Breeding Engineering Technology Research Center  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4854-6590","authenticated-orcid":false,"given":"Shijie","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science Agricultural University of Hebei  Baoding Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188278"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.21495"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2016.1184805"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11658-010-0041-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.13247"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-015-0143-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.250704.119"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa815"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.030403"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2008.01.009"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-020-00792-6"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv960"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/15592294.2014.970052"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-018-0092-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3766"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006427"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005644"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711-021-00674-7"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-020-02914-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-022-02745-4"}],"container-title":["Animal 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