{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:45:19Z","timestamp":1761180319202,"version":"build-2065373602"},"reference-count":76,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100021778","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PICT 2014\u20100396","PICT 2018\u201001916"],"award-info":[{"award-number":["PICT 2014\u20100396","PICT 2018\u201001916"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008777","name":"Fundaci\u00f3n Bunge y Born","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008777","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Genetics"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Llama breeding is an important source of economic income for Andean communities, thus ensuring llama biodiversity is fundamental to making breeding decisions that promote sustainable production. Despite this, there is limited information on the genetic diversity of llama populations in Argentina. Moreover, some llama herds in the northwest region of the country exhibit low reproductive efficiency and a high incidence of congenital abnormalities. In this study, we used genotyping by sequencing, a genome\u2010wide approach, to estimate the genetic diversity of Argentine llama populations, assess their conservation status and inbreeding levels, and discuss the impacts on breeding. Overall, our results indicate that current llama populations in the northwest present moderate to high genetic diversity, although a recent reduction in population size was detected for two of them. Population structure was subtle, although three clusters, with some substructure, were recognized. The inbreeding coefficient values were similar for all populations showing moderate to high inbreeding levels. There was a predominance of short homozygous segments, which is indicative of ancient consanguinity. However, recent autozygosity events were evidenced in some llamas with congenital disorders. Thus, we investigated the chromosomal region to find potential candidate genes for such traits. The genes\n                    <jats:italic>MYO15A<\/jats:italic>\n                    and\n                    <jats:italic>USH1G<\/jats:italic>\n                    are proposed as candidates for pigmentation\u2010associated deafness, although further research is needed. This study establishes an initial step towards understanding the genetic diversity of Argentine llamas, highlighting the necessity of reducing current inbreeding levels and implementing continuous monitoring to improve breeding decisions and support a sustainable production system for the species.\n                  <\/jats:p>","DOI":"10.1111\/age.70053","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T12:49:43Z","timestamp":1760014183000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genotyping by sequencing\u2010based genetic insights into Argentine llama populations and breeding impacts"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5849-2904","authenticated-orcid":false,"given":"M.","family":"Anello","sequence":"first","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Molecular, Instituto Multidisciplinario de Biolog\u00eda Celular (IMBICE) CONICET\u2010UNLP\u2010CIC  La Plata, Buenos Aires Argentina"},{"name":"Grupo de Investigaciones en Ecolog\u00eda y Fisiolog\u00eda de Fauna Silvestre Instituto de Investigaciones en Biodiversidad y Medio Ambiente (GIEFAS\u2010INIBIOMA\u2010CONICET\u2010UNCo)  Neuqu\u00e9n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Muzzio","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Molecular, Instituto Multidisciplinario de Biolog\u00eda Celular (IMBICE) CONICET\u2010UNLP\u2010CIC  La Plata, Buenos Aires Argentina"},{"name":"Facultad de Ciencias Naturales y Museo Universidad Nacional de La Plata  La Plata, Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. B.","family":"Silbestro","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Molecular, Instituto Multidisciplinario de Biolog\u00eda Celular (IMBICE) CONICET\u2010UNLP\u2010CIC  La Plata, Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. S.","family":"Daverio","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Molecular, Instituto Multidisciplinario de Biolog\u00eda Celular (IMBICE) CONICET\u2010UNLP\u2010CIC  La Plata, Buenos Aires Argentina"},{"name":"Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata  La Plata, Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Unzaga","sequence":"additional","affiliation":[{"name":"Laboratorio Sat\u00e9lite Forense\u2010MP\u2010Poder Judicial de Catamarca  Catamarca Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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Guay K.P. Sold\u00e0 T. Hitchman C.J. Vasiljevi S. Modenutti C.P.et\u00a0al. (2023)Rescue of secretion of a rare\u2010disease associated mis\u2010folded mutant glycoprotein in UGGT1 knock\u2010out mammalian cells.BioRxivPrepr.https:\/\/doi.org\/10.1101\/2023.05.30.542711","DOI":"10.1101\/2023.05.30.542711"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711\u2010016\u20100196\u2010y"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.05.010"},{"key":"e_1_2_9_73_1","first-page":"74","article-title":"Brainstem auditory evoked responses and ophthalmic findings in llamas and alpacas in eastern Canada","volume":"47","author":"Webb A.A.","year":"2006","journal-title":"The Canadian Veterinary Journal"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg051"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.2307\/2408641"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.565216"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12881\u2010019\u20100790\u20102"}],"container-title":["Animal Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/age.70053","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T15:12:20Z","timestamp":1761145940000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/age.70053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":76,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/age.70053"],"URL":"https:\/\/doi.org\/10.1111\/age.70053","archive":["Portico"],"relation":{},"ISSN":["0268-9146","1365-2052"],"issn-type":[{"type":"print","value":"0268-9146"},{"type":"electronic","value":"1365-2052"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70053"}}