{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T01:02:46Z","timestamp":1773622966280,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T00:00:00Z","timestamp":1526601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["TOVAG 1130578"],"award-info":[{"award-number":["TOVAG 1130578"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The pineal gland is a neuroendocrine organ associated with photoperiodic regulation in mammals. The aim of this study was to evaluate the pineal gland at the pre\u2010pubertal, pubertal and post\u2010pubertal periods by means of morphology and stereology. The study examined at total of 24 ovine pineal glands collected from healthy female Akkaraman breed. Thick sections (40\u00a0\u03bcm) were cut and treated with synaptophysin. Following each thick section, six consecutive sections at a thickness of 5\u00a0\u03bcm were cut. Each thin section was stained with one of the following dyes: Crossman\u2019s modified triple dye, glial fibrillary acidic protein (<jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content>), melatonin marker, periodic acid\u2013Schiff, Von Kossa and Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content>. The pineal gland volume was measured using Cavalieri\u2019s method. The optical fractionator was used to estimate the total number of pinealocytes. The percentage of parenchyma and connective tissue and degree of vascularization were estimated by the area fraction fractionator method. The pineal gland volumes in the pre\u2010pubertal, pubertal and post\u2010pubertal groups were 7.53\u00a0\u00b1\u00a01.715\u00a0mm<jats:sup>3<\/jats:sup>, 11.20\u00a0\u00b1\u00a01.336\u00a0mm<jats:sup>3<\/jats:sup> and 17.75\u00a0\u00b1\u00a01.188\u00a0mm<jats:sup>3<\/jats:sup>, respectively (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.5). The number of pinealocytes in the pre\u2010pubertal, pubertal and post\u2010pubertal groups was 3,244,000\u00a0\u00b1\u00a0228,076, 4,438,000\u00a0\u00b1\u00a0243,610, 7,381,766\u00a0\u00b1\u00a0406,223, respectively (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The glands of the post\u2010pubertal group contained the highest amount of connective tissue (11.49\u00a0\u00b1\u00a02.103%; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.5) and the largest <jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content> staining area (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The melatonin staining density was the highest in the pubertal group. The density of lipofuscin staining was higher in the pubertal and post\u2010pubertal groups.<\/jats:p>","DOI":"10.1111\/ahe.12359","type":"journal-article","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T11:13:29Z","timestamp":1526642009000},"page":"338-345","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Morphology and quantification of sheep pineal glands at pre\u2010pubertal, pubertal and post\u2010pubertal periods"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7619-1913","authenticated-orcid":false,"given":"Durmu\u015f","family":"Bolat","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Anatomy K\u0131r\u0131kkale University  Yahsihan K\u0131r\u0131kkale Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayt\u00fcl","family":"K\u00fcr\u00fcm","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Histology and Embryology K\u0131r\u0131kkale University  Yahsihan K\u0131r\u0131kkale Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u0131la","family":"Canpolat","sequence":"additional","affiliation":[{"name":"Faculty of Engineering Multidisciplinary Laboratories \u0130zmir Economy University  Balcova \u0130zmir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2002.02110.x"},{"key":"e_1_2_6_3_1","volume-title":"Applied animal reproduction","author":"Bearden H. 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