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However, little is known about the remodelling of the cervical tissue during the prepubertal development of the lambs. To obtain histochemical and biochemical evidence about the nature of the prepubertal development of the cervix of the ewe, cervices of Corriedale lambs obtained at 0, 1, 2, 4, 6 and 8\u00a0months of age (<jats:italic>n<\/jats:italic>\u00a0=\u00a05 to 6 in each) were processed. Neutral and acidic glycosaminoglycans (by PAS\u2010Alcian stain) were weakly in the cervical stroma and not shown change during the development, whereas the percentage volume of fibrillar collagen (by van Gieson stain) increases throughout the experimental period in the superficial fold stroma and deep wall stroma (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The relative cervical weight (g\/kg of body weight) and the collagen concentration (by spectrophotometry, mg\/mg wet tissue) showed an early decreasing phase from months 0 to 4 and a later increasing phase from months 4 to 8 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The latent form of matrix metalloproteinase\u20102 (MMP\u20102) detected by gelatin zymography (ng\/mg protein) decreased from months 0 to 2 and increased from months 4 to 8, whereas the activated form decreased from months 0 to 2, remained low until month 6 and then recovered on month 8 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001). Data suggest that the relative cervical weight biphasic pattern during the development is related to MMP\u20102\u2010dependent changes in the collagen content.<\/jats:p>","DOI":"10.1111\/ahe.12441","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T06:09:13Z","timestamp":1553494153000},"page":"306-314","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Collagen, glycosaminoglycans and matrix metalloproteinase\u20102 and metalloproteinase\u20109 in the cervix of the ewe during prepubertal development"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3377-8375","authenticated-orcid":false,"given":"Marcelo","family":"Rodr\u00edguez\u2010Pi\u00f1\u00f3n","sequence":"first","affiliation":[{"name":"Biochemistry, Department of Molecular and Cellular Biology Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Casuriaga","sequence":"additional","affiliation":[{"name":"Biochemistry, Department of Molecular and Cellular Biology Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Genovese","sequence":"additional","affiliation":[{"name":"Histology and Embryology, Department of Morphology and Development, Veterinary Faculty Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gonzalo","family":"Garc\u00eda\u2010Barcelo","sequence":"additional","affiliation":[{"name":"Biochemistry, Department of Molecular and Cellular Biology Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Alcaide","sequence":"additional","affiliation":[{"name":"Histology and Embryology, Department of Morphology and Development, Veterinary Faculty Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Bielli","sequence":"additional","affiliation":[{"name":"Histology and Embryology, Department of Morphology and Development, Veterinary Faculty Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.1.26"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.7.4008"},{"key":"e_1_2_7_4_1","first-page":"11249","article-title":"Effects of mifepristone and progesterone on collagen synthesis uterine cervix in the human","volume":"5","author":"Bokstr\u00f6m H.","year":"1995","journal-title":"Contraception"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(96)01492-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.06.023"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2740320603"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2012-1329"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90182-I"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(90)90060-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(03)00136-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.1.27"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.02.018"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12312"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.07.014"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.9.8387"},{"key":"e_1_2_7_17_1","first-page":"103","article-title":"The biochemistry and physiology of the uterine cervix during gestation and parturition","volume":"3","author":"Leppert P. 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