{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:09:01Z","timestamp":1765807741870,"version":"3.40.5"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009088","name":"Veterin\u00e4rmedizinische Universit\u00e4t Wien","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009088","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Angiogenesis and sepsis\u2010related equine laminitis have several features in common. Both events can be induced by endotoxin (lipopolysaccharide\u2014 LPS) and both are associated with increased expression of the enzyme cyclooxygenase (COX), of which two isoforms (COX\u20101 and COX\u20102) exist. To examine the causal relationship between LPS exposure and COX expression and to investigate the tissue distribution of COX in the LPS\u2010exposed tissue, the technique of extracorporeal haemoperfusion of isolated equine forelimbs was utilized. Perfusion was performed for 10\u00a0hr under physiological conditions (control\u2010perfused limbs,<jats:italic>n<\/jats:italic>\u00a0=\u00a05) and with addition of 80\u00a0ng\/L of endotoxin (LPS\u2010perfused limbs;<jats:italic>n<\/jats:italic>\u00a0=\u00a05). After perfusion, samples of lamellar tissue were collected from the dorsal aspect of the hoof wall. Additional control samples were collected from three non\u2010perfused limbs. Immunohistochemical analysis was performed using antibodies against COX\u20101 and COX\u20102, and intensity of immunohistochemical staining was scored for each isoform. In the lamellar tissue of control\u2010 and LPS\u2010perfused limbs, there was no significant difference in COX\u20101 staining intensity and distribution, whereas COX\u20102 expression was significantly increased in LPS\u2010perfused limbs (especially in endothelial cells, fibroblasts and intravasal leucocytes as well as in epidermal basal cells at the base of the primary epidermal lamellae). These results suggest that COX\u20102 and its metabolites are involved in the initiation of pathological changes seen in sepsis\u2010associated events such as sepsis\u2010related laminitis. In such cases, COX\u20102 could therefore be an important therapeutic target; however, early therapy may be required as increase in COX\u20102 expression occurs within 10\u00a0hr after LPS exposure.<\/jats:p>","DOI":"10.1111\/ahe.12520","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T15:45:15Z","timestamp":1574869515000},"page":"597-605","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Endotoxin\u2010induced changes in expression of cyclooxygenase isoforms in the lamellar tissue of extracorporeally haemoperfused equine limbs"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9466-354X","authenticated-orcid":false,"given":"Bianca","family":"Patan\u2010Zugaj","sequence":"first","affiliation":[{"name":"Institute of Topographic Anatomy University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Egerbacher","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Veterinary Medicine Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresia F.","family":"Licka","sequence":"additional","affiliation":[{"name":"Equine University Clinic University of Veterinary Medicine Vienna Vienna Austria"},{"name":"Royal (Dick) School of Veterinary Studies University of Edinburgh Roslin UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/970890"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041520127"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2006.tb00721.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.11.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.10.024"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.1.87"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-014-0221-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1989.tb05650.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2746\/0425164044877125"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(02)00011-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.09.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-018-2788-z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2010.00122.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.11.1205"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.11.003"},{"key":"e_1_2_7_17_1","first-page":"191","article-title":"Endotoxin is angiogenic","volume":"75","author":"Mattsby\u2010Baltzer I.","year":"1994","journal-title":"International Journal of Experimental Pathology"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9523"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2011.02.026"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/inp.k4485"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516407X177448"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.230.6.885"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.70.5.669"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.79.9.986"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.73.9.1462"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.75.9.842"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-01-0288"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.2042-3306.1998.tb05131.x","article-title":"Equine laminitis: Its development coincides with increased sublamellar blood flow","volume":"26","author":"Pollitt C. 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