{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:40:16Z","timestamp":1788910816677,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of live animals for educational purposes is an old practice that is still employed in teaching and research institutions. However, there are several objections to this practice, whether for ethical or humanitarian reasons. Surgical techniques teaching using anatomical pieces and\/or preserved cadavers promotes greater learning efficiency, provides exercise repetition and increases the confidence and satisfaction of the students when compared to the use of live animals. The current work aimed to analyse the feasibility of using fresh swine urinary bladder and small intestines (jejunum), obtained from slaughterhouses, fixed in 99.8% ethyl alcohol (EA) and preserved in sodium chloride hypersaturated solution (SCHS) at 30%, for 7, 14 and 21\u00a0days, as an alternative method for surgical skills training (SST). Swine viscera, fixed in EA and preserved in SCHS, presented a realistic appearance, absence of odour and maintained the viable morphological characteristics during the performance of the operative techniques. Preservation solutions had low cost, were easy to acquire and did not offers risks to human health. Therefore, urinary bladders and small intestines fixed in 99.8% EA for 30\u00a0days and maintained in 30% SCHS at different periods were demonstrated as a good viable option as a preservation method for surgical skills training.<\/jats:p>","DOI":"10.1111\/ahe.12739","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T14:47:44Z","timestamp":1632926864000},"page":"996-1006","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Swine viscera preservation in hypersaturated salt solution after alcohol fixation as a preparation method for educational purposes"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6964-0589","authenticated-orcid":false,"given":"Julia Belotto","family":"Guaran\u00e1","sequence":"first","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4053-9161","authenticated-orcid":false,"given":"Alois Foltran","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8810-2815","authenticated-orcid":false,"given":"Ricardo de Francisco","family":"Strefezzi","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1860-8623","authenticated-orcid":false,"given":"Fabr\u00edcio Singaretti","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Animal Morphology and Physiology School of Agrarian and Veterinary Sciences S\u00e3o Paulo State University (Unesp) Jaboticabal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4897-2848","authenticated-orcid":false,"given":"Luciana Cristina","family":"Machado","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8224-5022","authenticated-orcid":false,"given":"Carlos Eduardo","family":"Ambr\u00f3sio","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4275-0358","authenticated-orcid":false,"given":"Renata Gebara Sampaio","family":"D\u00f3ria","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-0367","authenticated-orcid":false,"given":"Silvio Henrique de","family":"Freitas","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Food Engineering Department of Veterinary Medicine University of S\u00e3o Paulo (USP) Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ca.22585"},{"issue":"23","key":"e_1_2_9_3_1","first-page":"68","article-title":"Alternatives to the use of laboratory animals in veterinary education","volume":"1","author":"Baumans V.","year":"2006","journal-title":"Altex"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12565-019-00508-z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782002000600016"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.17.01256"},{"issue":"1","key":"e_1_2_9_7_1","first-page":"126","article-title":"Sistema educacional hiperm\u00eddia como ferramenta de apoio ao ensino de Medicina Veterin\u00e1ria","volume":"13","author":"Costa Neto J.","year":"2010","journal-title":"Ci\u00eancia Veterin\u00e1ria Nos Tr\u00f3picos"},{"issue":"32","key":"e_1_2_9_8_1","first-page":"16","article-title":"Bastidor Aplicado Ao Ensino Da T\u00e9cnica Cir\u00fargica Veterin\u00e1ria \u2010 S\u00edntese Dos Tecidos","volume":"10","author":"Costa Neto J. 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