{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:23:27Z","timestamp":1790483007205,"version":"4.1.0"},"reference-count":81,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009088","name":"Veterin\u00e4rmedizinische Universit\u00e4t Wien","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009088","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Here, we describe a workflow for high\u2010detail microCT imaging of formalin\u2010fixed and paraffin\u2010embedded (FFPE) equine embryos recovered on Day 34 of pregnancy (E34), a period just before placenta formation. The presented imaging methods are suitable for large animals' embryos with intention to study morphological and developmental aspects, but more generally can be adopted for all kinds of FFPE tissue specimens. Microscopic 3D imaging techniques such as microCT are important tools for detecting and studying normal embryogenesis and developmental disorders. To date, microCT imaging of vertebrate embryos was mostly done on embryos that have been stained with an X\u2010ray dense contrast agent. Here, we describe an alternative imaging procedure that allows to visualize embryo morphology and organ development in unstained FFPE embryos. Two aspects are critical for high\u2010quality data acquisition: (i) a proper sample mounting leaving as little as possible paraffin around the sample and (ii) an image filtering pipeline that improves signal\u2010to\u2010noise ratio in these inherently low\u2010contrast data sets. The presented workflow allows overview imaging of the whole embryo proper and can be used for determination of organ volumes and development. Furthermore, we show that high\u2010resolution interior tomographies can provide virtual histology information from selected regions of interest. In addition, we demonstrate that microCT scanned embryos remain intact during the scanning procedure allowing for a subsequent investigation by routine histology and\/or immunohistochemistry. This makes the presented workflow applicable also to archival paraffin\u2010embedded material.<\/jats:p>","DOI":"10.1111\/ahe.12834","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T08:30:29Z","timestamp":1658133029000},"page":"611-623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["An optimized workflow for <scp>microCT<\/scp> imaging of formalin\u2010fixed and paraffin\u2010embedded (<scp>FFPE<\/scp>) early equine embryos"],"prefix":"10.1111","volume":"51","author":[{"given":"Stephan","family":"Handschuh","sequence":"first","affiliation":[{"name":"VetCore Facility for Research\/Imaging Unit University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina T. C.","family":"Okada","sequence":"additional","affiliation":[{"name":"Platform Artificial Insemination and Embryo Transfer Department for Small Animals and Horses University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Walter","sequence":"additional","affiliation":[{"name":"VetCore Facility for Research\/VetBiobank University of Veterinary Medicine Vienna  Vienna Austria"},{"name":"Institute of Morphology University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Aurich","sequence":"additional","affiliation":[{"name":"Platform Artificial Insemination and Embryo Transfer Department for Small Animals and Horses University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Gl\u00f6smann","sequence":"additional","affiliation":[{"name":"VetCore Facility for Research\/Imaging Unit University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1358"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104\u2010015\u20100048\u20106"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0950339"},{"key":"e_1_2_8_5_1","volume-title":"Developmental horizons and measurements useful for age determination of equine embryos and fetuses","author":"Bergin W. C.","year":"1968"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD11132"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.141"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2007.07.007"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1720862115"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102818.2008.02142.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cirp.2014.05.011"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.10.008"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927618014320"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0189"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19356"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30385"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2113835118"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335\u2010017\u20109723\u20106"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.04.014"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.187633"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/vop.12920"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927614013063"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/app9183826"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12449"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2018.00501"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742\u20109994\u201010\u201044"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.198291"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0800395"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1259\/bjr.20170113"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03026336"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020061"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.86375"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.181842"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2017.03.006"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2019.05.004"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.5852\/ejt.2019.522"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001980170022"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08920"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1179\/1743280413y.0000000023"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13355"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/mollus\/eym044"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106793\u20109\u201011"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21857"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2011.10.002"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jmi.12385"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201036067\u2010y"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD20116"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/jmi.12013"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2507"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top116"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915\u2010020\u20100753\u20102"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.6.040803.140130"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010bioeng\u2010071910\u2010124717"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2021.109385"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.4172\/2157\u20107099.1000240"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1100\/tsw.2007.125"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155410394857"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862\u2010018\u20101206\u20101"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126230"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155412473757"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1159\/000442387"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.6.040803.140210"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068206"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23741"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1179\/095066099101528261"},{"key":"e_1_2_8_66_1","volume-title":"Microcomputed tomography: methodology and applications","author":"Stock S. R.","year":"2020"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223\u2010002\u20101037\u20102"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00339\u2010006\u20103507\u20102"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.17629\/www.diagnosticpathology.eu"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2019.05.043"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801678115"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejcn.2015.117"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtadv.2020.100084"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphy.2020.00047"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10074"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010420138\u20105.00011\u20102"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd5040058"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073491"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.107722"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084321"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00091.2010"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.4003\/006.036.0205"}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.12834","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ahe.12834","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.12834","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T06:57:23Z","timestamp":1692773843000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ahe.12834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,18]]},"references-count":81,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1111\/ahe.12834"],"URL":"https:\/\/doi.org\/10.1111\/ahe.12834","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"value":"0340-2096","type":"print"},{"value":"1439-0264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,18]]},"assertion":[{"value":"2022-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}