{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T23:12:18Z","timestamp":1779318738599,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T00:00:00Z","timestamp":1660435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although formaldehyde is the most widely used and largely available fixative for preserving cadavers through decomposition prevention, it promotes darkening and weight gain, in addition to being considered carcinogenic. Ethyl alcohol has been proven a potential substitute to formaldehyde due to its effectiveness in tissue penetration, thus preventing proliferation of microorganisms; however, it can only be used alone for fixation of small parts. In view of such fixatives limitations, saturated salt solution has been widely employed based on its antimicrobial effect and ability to maintain tissue similar to the original one, in addition to exerting no hazardous effects as there is no evaporation of harmful substances. This research aimed to observe anatomical brain behaviour submitted to formaldehyde, alcohol, and saturated salt solution as fixatives. Fixatives were tested in 15 adult Wistar rats\u2019 brain, submerged in 10\u00a0ml of intended solution after removal for 4\u2009weeks. Weight of the brains fixed in saturated salt did not change over the weeks. However, the weight of formaldehyde\u2010fixed brains increased and the weight in alcohol\u2010fixed brains decreased; in addition, modifications in all solutions measures were also observed. Alcohol provides a peculiar dehydrating effect as formaldehyde clearly increases the length of the pieces. Thus, since the saturated salt solution showed no important adjustment over the experimental time, it proved an efficient alternative for replacing formaldehyde and alcohol as fixative solutions of anatomical study of the brain.<\/jats:p>","DOI":"10.1111\/ahe.12852","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T07:14:15Z","timestamp":1660461255000},"page":"740-745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Anatomo\u2010comparative study of formaldehyde, alcohol, and saturated salt solution as fixatives in Wistar rat brains"],"prefix":"10.1111","volume":"51","author":[{"given":"Camila","family":"Martins\u2010Costa","sequence":"first","affiliation":[{"name":"Laboratory of Scientific Acupuncture and Infectious\u2010Morphological Investigations (LACIIM), Institute of Biological and Health Sciences Federal University of MatoGrosso  Barra do Gar\u00e7as Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thauane Carvalho","family":"Nunes","sequence":"additional","affiliation":[{"name":"Laboratory of Scientific Acupuncture and Infectious\u2010Morphological Investigations (LACIIM), Institute of Biological and Health Sciences Federal University of MatoGrosso  Barra do Gar\u00e7as Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2754-7722","authenticated-orcid":false,"given":"Luana dos","family":"Anjos\u2010Ramos","sequence":"additional","affiliation":[{"name":"Laboratory of Scientific Acupuncture and Infectious\u2010Morphological Investigations (LACIIM), Institute of Biological and Health Sciences Federal University of MatoGrosso  Barra do Gar\u00e7as Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ca.22585"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12160"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638716672252"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23894"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100\u2010736X2013000500022"},{"key":"e_1_2_8_7_1","volume-title":"Tutankamon os Tesouros de sua Tumba","author":"Gon\u00e7alves L. 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