{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:02:01Z","timestamp":1790168521852,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Di(<jats:italic>n<\/jats:italic>\u2010butyl) phthalate (DBP) is considered a substance of serious concern because of its reproductive toxicity and endocrine\u2010disrupting properties. Exposure to DBP causes morphological and functional changes in the male reproductive system of birds and mammals. However, there are no detailed reports on the effects of DBP on the Sertoli cell and junctional complexes of the blood\u2013testis barrier (BTB) in birds. The present study investigated dose\u2010related ultrastructural changes in Sertoli cells and junctional complexes of the BTB in adult Japanese quail (<jats:italic>Coturnix coturnix japonica<\/jats:italic>) exposed to DBP prior to puberty. A total of 25 Japanese quail were used for the study. Exposure to DBP doses of 50, 200 and 400\u2009mg DBP\/kg\/d caused dose\u2010related ultrastructural changes in junctional complexes including dilation and separation, while disruption of cytoplasmic membranes and mitochondria was observed in Sertoli cells. There was a significant difference in the sum of vacuoles, vacuole diameter, nuclear width, nuclear length, nuclear area, sum of damaged spherical mitochondria, width of elongated mitochondria and the sum of damaged elongated mitochondria among the five treatment groups (<jats:italic>p<\/jats:italic>\u00a0\u02c2\u00a00.05). Prepubertal exposure to DBP at doses of 50, 200 and 400\u2009mg DBP\/kg\/d for 30\u2009days led to adverse effects in the adult male Japanese quail reproductive system by inducing structural changes in the Sertoli cells and junctional complexes. Such changes might disrupt the BTB and potentially interfere with spermatogenesis. Results indicated that the Sertoli cell is sensitive to DBP exposure and might be an important cellular target for DBP\u2010induced testicular toxicity.<\/jats:p>","DOI":"10.1111\/ahe.12902","type":"journal-article","created":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T04:57:25Z","timestamp":1673067445000},"page":"411-420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effect of di(<i>n<\/i>\u2010butyl) phthalate on the blood\u2013testis barrier during puberty onset"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7905-9758","authenticated-orcid":false,"given":"Reneilwe A.","family":"Molele","sequence":"first","affiliation":[{"name":"Department of Production Animal Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6285-105X","authenticated-orcid":false,"given":"Musa","family":"Zakariah","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"},{"name":"Department of Veterinary Anatomy, Faculty of Veterinary Medicine PMB 1069 University of Maiduguri  Maiduguri Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0830-2209","authenticated-orcid":false,"given":"Mohammed I. 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