{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T19:10:52Z","timestamp":1781118652537,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,4]],"date-time":"2023-06-04T00:00:00Z","timestamp":1685836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Avian ceca play an important role in liquid absorption, cellulose digestion, and defensive mechanism. This study aims to demonstrate histological and histochemical characteristics of developing chicken cecum. For this purpose, 10 embryos on the 18th day of incubation, 10 chicks on hatching day and 10 chicks on the seventh day post\u2010hatching were used. The histological sections prepared from proximal, middle, and distal parts of cecum were stained with Crossmon's triple stain, periodic\u2010acid Schiff, Alcian blue (pH\u20092.5), Masson\u2010Fontana's argentaffin silver stain and Gordon and Sweets's silver stain. Alpha\u2010naphthyl acetate esterase (ANAE) and acid phosphatase (ACP\u2010ase) were also demonstrated in the sections. In the proximal part, although the villi were rudimentary on the 18th day of incubation, well\u2010developed villi were seen at seventh day post\u2010hatching. In middle and distal parts, while it was seen that rudimentary folds appeared on the 18th day of incubation, mucosal folds were prominent and short villi were formed on the hatching day and seventh day post\u2010hatching. Goblet cells and enteroendocrine cells were detected from the 18th day of incubation. The lymphoid follicles supported with reticular fibres were seen on the seventh day post\u2010hatching in proximal cecum wall. While ACP\u2010ase (+) lymphocytes were rarely observed, more ANAE (+) lymphocytes were in lymphoid follicles. As a result, development of cecum in chickens has been demonstrated by histological techniques in this study.<\/jats:p>","DOI":"10.1111\/ahe.12936","type":"journal-article","created":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T16:29:28Z","timestamp":1686068968000},"page":"778-788","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Histological and histochemical characteristics of the developing chicken (<i>Gallus gallus<\/i> domesticus) cecum"],"prefix":"10.1111","volume":"52","author":[{"given":"Ya\u011fmur Raziye","family":"Ko\u00e7ak","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Sel\u00e7uk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u011fba","family":"\u00d6zayd\u0131n","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Sel\u00e7uk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201052335\u2010x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927619000655"},{"issue":"3","key":"e_1_2_8_4_1","first-page":"453","article-title":"A histochemical study of the stomach and intestine of the chicken","volume":"92","author":"Aitken R. N. C.","year":"1958","journal-title":"Journal of Anatomy"},{"key":"e_1_2_8_5_1","first-page":"206","volume-title":"Manual of histological techniques and their diagnostic application","author":"Bancroft J. D.","year":"1994"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/10.6.741"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.1980.tb08712.x"},{"key":"e_1_2_8_8_1","first-page":"119","volume-title":"The theory and practice of histological techniques","author":"Bradbury P.","year":"1990"},{"key":"e_1_2_8_9_1","first-page":"93","article-title":"The avian cecum: A review","volume":"107","author":"Clench M. H.","year":"1995","journal-title":"The Wilson Journal of Ornithology"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090690105"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/73.6.1131S"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071669108417394"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.9790\/2380\u20100121316"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19960194"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/195297a0"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20100185(199802)250:2<182::AID\u2010AR8>3.0.CO;2\u20105"},{"issue":"1","key":"e_1_2_8_17_1","first-page":"25","article-title":"The effect of anti\u2010bursa serum (ABS) on the intensity of acid phosphatase reaction in bursa dependent structures of the spleen and on the level of antibodies in the blood serum","volume":"34","author":"Graczyk S.","year":"1994","journal-title":"Archivum Veterinarium Polonicum"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574\u2010019\u20100168\u20108"},{"issue":"2","key":"e_1_2_8_19_1","first-page":"96","article-title":"Prepubertal and pubertal caecal wall histology injapanese quails (Coturnix coturnix japonica.)","volume":"16","author":"Hamedi S.","year":"2013","journal-title":"Bulgarian Journal of Veterinary Medicine"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2011.638391"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.0190003"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.111.309"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469\u20107580.1998.19220293.x"},{"issue":"1","key":"e_1_2_8_24_1","first-page":"70","article-title":"Tissue localization of T lymphocytes by the histochemical demonstration of acid alpha\u2010naphthyl acetate esterase","volume":"39","author":"Knowles D. M.","year":"1978","journal-title":"Laboratory Investigation"},{"issue":"10","key":"e_1_2_8_25_1","first-page":"1011","article-title":"Microanatomical studies on the caecum of Japanese quail","volume":"79","author":"Kumary U.","year":"2009","journal-title":"Indian Journal of Animal Sciences"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050480212"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00347739"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.33762\/bvetr.2009.55203"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.5455\/ijlr.20200417041833"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41232\u2010018\u20100072\u2010y"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3923\/javaa.2012.2936.2944"},{"issue":"1","key":"e_1_2_8_32_1","first-page":"85","article-title":"Comparative histological studies on the caeca of goat, pig, dog andbroiler chicken","volume":"56","author":"Paul S. R.","year":"2017","journal-title":"Indian Journal of Animal Health"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100442.1987.tb00295.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3844\/ajavsp.2007.50.54"},{"key":"e_1_2_8_35_1","first-page":"99","article-title":"Alpha\u2010naphthyl acetate esterase activity in the spleen, lymph nodes and conjunctiva associated lymphoid tissues of camels (Camelusdromedarius)","volume":"156","author":"Sand\u0131kc\u0131 M.","year":"2005","journal-title":"Revue De MedecineVeterinaire"},{"issue":"1","key":"e_1_2_8_36_1","first-page":"169","article-title":"Enzyme differentiation of lymphocyte subpopulations in section of human lymph nodes, tonsils and periodontal disease","volume":"32","author":"Seymour G. J.","year":"1978","journal-title":"Clinical and Experimental Immunology"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1079\/WPS20010030"},{"issue":"3","key":"e_1_2_8_38_1","first-page":"75","article-title":"Histochemical studies of acid phosphatase in the bursa\u2010dependent lymphoid tissue of the spleen of chickens after bursectomy and stimulation with ovine erythrocytes","volume":"30","author":"Slowik J.","year":"1990","journal-title":"Polskie Archiwum Weterynaryjne"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01740778"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660310001618352"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.9775\/kvfd.2013.9642"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933913000287"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/17.4.238"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781845931803.0029"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.2.320"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.11.1747"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071669888773"}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.12936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T10:22:08Z","timestamp":1694600528000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ahe.12936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,4]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/ahe.12936"],"URL":"https:\/\/doi.org\/10.1111\/ahe.12936","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"value":"0340-2096","type":"print"},{"value":"1439-0264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,4]]},"assertion":[{"value":"2023-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}