{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:43:23Z","timestamp":1787013803745,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T00:00:00Z","timestamp":1689033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004686","name":"Deanship of Scientific Research, King Faisal University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lectins are carbohydrate\u2010binding proteins that are highly selective for sugar groups on other molecules. Siglec5 is a cell\u2010surface lectin that belongs to the sialic acid\u2010binding Ig\u2010like lectins (Siglecs) and acts as a suppressor of immune responses. In this study, immunohistochemistry, western blotting and quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) were used to detect the expression of Siglec5 in the male reproductive tract of dromedary camels during the rutting season. Siglec5 displayed strong immunostaining in the cranial and caudal testicular regions and moderate immunostaining in the rete testis. Different parts of the epididymis showed varying immunoreactions to Siglec5. The spermatozoa in the testes and epididymis also showed positive immunostaining for Siglec5, whereas, the vas deferens showed negative immunostaining for the protein. The results obtained by western blotting confirmed the immunohistochemical detection of the protein in the testicular and epididymal tissues. The results of qRT\u2010PCR showed that Siglec mRNA was expressed differently in each part of the testis and epididymis; the highest levels of expression were observed in the caudal part of the testis and in the head of the epididymis. In conclusion, the present study revealed that Siglec5 is mainly located in the testis and epididymis, where sperm production and maturation occur. Therefore, this protein may play an essential role in the development, maturation and protection of camel sperm.<\/jats:p>","DOI":"10.1111\/ahe.12944","type":"journal-article","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T07:29:56Z","timestamp":1689060596000},"page":"874-881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immunolocalization and expression of Siglec5 protein in the male reproductive tract of dromedary camel during rutting season"],"prefix":"10.1111","volume":"52","author":[{"given":"Khalid Mohamed","family":"Al\u00a0Khodair","sequence":"first","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Salem","family":"Moqbel","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelrahman Mohamed Ali","family":"Elseory","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"},{"name":"Department of Anatomy, Faculty of Veterinary Medicine University of Khartoum  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud Gamil","family":"Elsebaei","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"},{"name":"Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thnaian A.","family":"Al\u2010Thnaian","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7239-7612","authenticated-orcid":false,"given":"Mortada M. O.","family":"Elhassan","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine University of Bahri  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,11]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Al Khodair K.(2015).Identification and characterisation of siglec expression on sperm and in the male reproductive tract. Doctoral Thesis. University College Dublin. School of Veterinary Medicine.http:\/\/hdl.handle.net\/10197\/8556"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201017\u20100475"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20131853"},{"key":"e_1_2_8_5_1","unstructured":"Almhanna H. Carrington S. Gallagher M.E. Kilroy D. Irwin J.A. Lonergan P. Evans A. andReid C.J.(2019).Siglec expression on sperm and tissue from the male reproductive tract in mice and rats. 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