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This study aimed to compare four embalming solutions for high\u2010colour\u2010quality images of different tissues compared to the original image. Four equine left pelvic limbs were cut into metameres and divided equally for application of 10% formaldehyde, 96% glycerine, 33% hypersaturated NaCl solution and modified Larssen solution, respectively, which was maintained for 3\u2009days. After drying for 3\u2009days at room temperature, photographs were obtained at time 0 (T0), without any fixation process (original colour); time 1 (T1), immediately after removal from the solutions; and every 24\u2009h for 3\u2009days (T2\u2013T4). The image colour quality was investigated by digitally evaluating the cortical bone, tendon and bone marrow using histograms and CIEDE2000 as well as by 10 specialists in an online survey. CIEDE2000 and histograms revealed that all fixation solutions changed the original tissue colour at all the time points (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). According to the specialists, the 33% saline solution produced the best results compared to the original one. The modified Larssen solution demonstrated better results for the tendon, marrow and cortical bone at T3 (<jats:italic>p<\/jats:italic>\u2009=\u20090.0015). Considering the colour of digital images, the modified Larssen solution provided the best results; however, the visual evaluation by the specialists revealed the 33% saline solution as the best.<\/jats:p>","DOI":"10.1111\/ahe.12975","type":"journal-article","created":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T09:54:31Z","timestamp":1695117271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring alternatives for securing anatomical structures in capturing digital images: A comparative analysis"],"prefix":"10.1111","volume":"53","author":[{"given":"Daniela G.","family":"Turchenski","sequence":"first","affiliation":[{"name":"Graduate Program in Animal Science, School of Medicine and Life Sciences Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Acir J.","family":"Franco","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, School of Medicine and Life Sciences Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael G.","family":"Turchenski","sequence":"additional","affiliation":[{"name":"Computer Engineer Dijkstra Optimal Solution  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"La\u00eds C.","family":"Werner","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science, School of Medicine and Life Sciences Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saulo H.","family":"Weber","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science, School of Medicine and Life Sciences Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohan B.","family":"Gumiel","sequence":"additional","affiliation":[{"name":"Department of Computer Science Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0893-1654","authenticated-orcid":false,"given":"Pedro V.","family":"Michelotto","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science, School of Medicine and Life Sciences Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1109\/EMBC.2016.7591442"},{"key":"e_1_2_10_3_1","first-page":"2154","volume-title":"The visible human project","author":"Ackerman M. 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