{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:26:28Z","timestamp":1753878388000,"version":"3.41.2"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T00:00:00Z","timestamp":1695081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mouse embryos in the early\u2010implantation stage require manipulation under a microscope. While the extraction of DNA, RNA and proteins from a single sample allows for both determination of genetic type and analysis of gene expression, whole mount analysis is not possible. In this study, we explored the applicability of PCR using extraembryonic tissues, especially the decidual side tissue after isolating the embryos from implantation sites to establish a method for determining the genetic type of embryos. The implantation site was resected at each day from the date of vaginal plug confirmation, separated into embryos and deciduae. Genomic DNA were isolated separately from the embryos and the deciduae. PCR was performed using these genomic DNA, and the band patterns were compared after electrophoresis. As a result, we demonstrated that detecting embryo\u2010derived cells in the decidua allows determination of the sex and presence of transgenes without harming the mouse embryos themselves, from 8.5\u2009days of age. This method enables the determination of the genetic type of mouse embryos without damaging. This technique would expand the adaptations for analysis of mouse implanted embryos.<\/jats:p>","DOI":"10.1111\/ahe.12976","type":"journal-article","created":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T10:14:51Z","timestamp":1695118491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Verification of the efficacy of deciduae for determining the genetic type of mouse embryos"],"prefix":"10.1111","volume":"53","author":[{"given":"Hiroyuki","family":"Imai","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Iwamori","sequence":"additional","affiliation":[{"name":"Laboratory of Zoology, Graduate School of Agriculture Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tokuko","family":"Iwamori","sequence":"additional","affiliation":[{"name":"Laboratory of Zoology, Graduate School of Agriculture Kyushu University  Fukuoka Japan"},{"name":"Research Fellow of Japan Society for the Promotion of Science  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumito","family":"Matsuya","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Embryology, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Kano","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Embryology, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken Takeshi","family":"Kusakabe","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine Yamaguchi University  Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmo.75"},{"volume-title":"Manipulating the mouse embryo: A laboratory manual","year":"2014","author":"Behringer R.","key":"e_1_2_8_3_1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3791\/57785"},{"key":"e_1_2_8_5_1","first-page":"536","article-title":"A reagent for the single\u2010step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples","volume":"15","author":"Chomczynski P.","year":"1993","journal-title":"BioTechniques"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.21\u20100119"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.082768dh"},{"volume-title":"The Atlas of Mouse Development","year":"1995","author":"Kaufman M. H.","key":"e_1_2_8_8_1"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.03.035"},{"volume-title":"Mouse Phenotypes: A Handbook of Mutation Analysis","year":"2005","author":"Papaioannou V. E.","key":"e_1_2_8_10_1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044\u20105781(06)80049\u20106"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod12.1.41"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88418-4"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201050731\u2010x"}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.12976","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T11:50:56Z","timestamp":1705924256000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ahe.12976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,19]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/ahe.12976"],"URL":"https:\/\/doi.org\/10.1111\/ahe.12976","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"type":"print","value":"0340-2096"},{"type":"electronic","value":"1439-0264"}],"subject":[],"published":{"date-parts":[[2023,9,19]]},"assertion":[{"value":"2023-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12976"}}