{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T01:10:11Z","timestamp":1771463411199,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100018847","name":"Sel\u00e7uk University Research Foundation","doi-asserted-by":"publisher","award":["2014\u2010OYP\u2010023"],"award-info":[{"award-number":["2014\u2010OYP\u2010023"]}],"id":[{"id":"10.13039\/501100018847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stereology is a discipline that allows us to obtain quantitative information about the geometric structure of three\u2010dimensional objects. In this study, the volume of grey matter (GM), white matter (WM), and lateral ventricle (LV) of the cerebral hemispheres (CH) in sheep and goats were calculated. For this purpose, six healthy male sheep and goat brains (1\u20132\u2009years old) without any anomaly were used. Brains were fixed with 10% formaldehyde in the skull. The skull was opened using standard anatomical dissection methods, and the brains were carefully removed. Brain weight and volume were measured (using Archimedes' principle) after the meninges were removed. The cerebral hemispheres were separated from the other parts of the brain by a section made in front of the rostral colliculus. In the same way, the weight and volume of the cerebral hemispheres were measured. Afterward, the cerebral hemispheres were blocked with agar, and transversal cross sections (from rostral to caudal) with an average thickness of 3.42\u2009mm were taken from the cerebral hemispheres. Grey matter was stained with Berlin blue macroscopic staining method. The stained cross sections were scanned at 600 dpi resolution, and a point counting grid was placed on the images with the ImageJ software. Cavalieri's principle calculated the surface area and volume measurements of the grey matter, white matter, and lateral ventricle. GM, WM, and LV volumes in sheep and goat cerebral hemispheres were calculated as 54.94, 21.48 and 3.06\u2009mL in sheep, 57.46, 24.13 and 3.12\u2009mL in goats, respectively. The percentages of these structures in the total hemisphere volume were 71.83%, 28.17% and 4.00% in sheep, 70.42%, 29.58% and 3.82% in goats, respectively. Asymmetry was not observed in cerebral hemispheres in both species. A difference was found in the WM, LV and LV: CH ratios in the right\/left comparison of the goat (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In comparing sheep and goats, a significant difference was observed in WM right, WM left, WM total, CH left and CH total (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In conclusion, the cerebral hemispheres' grey matter and white matter ratio are frequently used to diagnose neurodegenerative diseases. In recent years, the increase in neurodegenerative disease models in farm animals has been enormous. It is thought that these values obtained from healthy animals in the current study will be important for such experimental studies in the future.<\/jats:p>","DOI":"10.1111\/ahe.12983","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:03:53Z","timestamp":1697094233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Calculation of cerebral hemispheres volume values (grey matter, white matter and lateral ventricle) of sheep and goat: A stereological study"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9354-3519","authenticated-orcid":false,"given":"Sedat","family":"Aydo\u011fdu","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Veterinary Medicine Sel\u00e7uk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7426-5325","authenticated-orcid":false,"given":"Emrullah","family":"Eken","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Veterinary Medicine Sel\u00e7uk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2014.11.026"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.06.013"},{"key":"e_1_2_10_4_1","volume-title":"Spatial statistics and digital image analysis","author":"Baddeley A.","year":"1991"},{"issue":"2","key":"e_1_2_10_5_1","first-page":"102","article-title":"Atlarda beyin hemisferlerine ait substantia grisea, alba ve ventriculus lateralis hacimlerinin Cavalieri prensibi kullan\u0131larak hesaplanmas\u0131","volume":"30","author":"Bahar S.","year":"2014","journal-title":"Eurasian Journal of Veterinary Sciences"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024\u20103205(94)00406\u20104"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahe.12359"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20103940(94)90589\u20104"},{"issue":"1","key":"e_1_2_10_9_1","first-page":"7","article-title":"Estimation of the reference volume, volume density and volume ratios by a stereological method: cavalieri's principle","volume":"22","author":"Canan S.","year":"2002","journal-title":"T\u00fcrkiye Klinikleri Journal of Medical Sciences"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2020.599701"},{"issue":"4","key":"e_1_2_10_11_1","first-page":"603","article-title":"Evaluation of cerebrum volume of children 1\u20105\u2009years old","volume":"25","author":"Cuce G.","year":"2014","journal-title":"Biomedical Research"},{"key":"e_1_2_10_12_1","volume-title":"Medisan Press","author":"Dursun N.","year":"2008"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731113001614"},{"key":"e_1_2_10_14_1","volume-title":"Textbook of veterinary anatomy","author":"Dyce K. M.","year":"2010"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/af\/vfz024"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24079"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.268970"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699\u20100463.1988.tb05320.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102818.1987.tb02837.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102818.1999.00457.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.10.086"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4324\/9780203006399"},{"issue":"1","key":"e_1_2_10_23_1","first-page":"1","article-title":"An overview of animal prion diseases","volume":"8","author":"Imran M.","year":"2011","journal-title":"Virology Journal"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.9775\/kvfd.2011.5401"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2005.01.005"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2017.06.002"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhr033"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.48550\/arXiv.2110.07711"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20104886(82)90053\u2010X"},{"key":"e_1_2_10_30_1","volume-title":"Schattauer","author":"K\u00f6nig H. E.","year":"2004"},{"key":"e_1_2_10_31_1","first-page":"36","article-title":"The major and minor sulci of the brain of the sheep","volume":"30","author":"Landacre F.","year":"1930","journal-title":"The Ohio Journal of Science"},{"key":"e_1_2_10_32_1","first-page":"191","article-title":"Comparative morphometry of the mammalian brain: Estimates of cerebral volumes and cortical surface areas obtained from macroscopic slices","volume":"172","author":"Mayhew T.","year":"1990","journal-title":"Journal of Anatomy"},{"issue":"1","key":"e_1_2_10_33_1","first-page":"177","article-title":"Stereological and allometric studies on mammalian cerebral cortex with implications for medical brain imaging","volume":"189","author":"Mayhew T.","year":"1996","journal-title":"Journal of Anatomy"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.2140\/pjm.2013.265.123"},{"key":"e_1_2_10_35_1","volume-title":"Principles and practices of unbiased stereology: An introduction for bioscientists","author":"Mouton P. R.","year":"2002"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197\u20104580(98)00080\u20103"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.831838"},{"key":"e_1_2_10_38_1","volume-title":"Lehrbuch der anatomie der haustiere band IV","author":"Nickel R.","year":"2004"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2015.00069"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2013.03.009"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.31590\/ejosat.697446"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468\u20101331.2000.00017.x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2017.06.020"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304\u20103940(00)01067\u20103"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.5566\/ias.v20.p9\u201013"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0720\u2010048X(02)00152\u20103"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijp.2006.253.255"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1984.tb02501.x"},{"key":"e_1_2_10_49_1","volume-title":"Koparatif veteriner anatomi sinir sistemi","author":"Tecirlio\u011flu S.","year":"1983"},{"key":"e_1_2_10_50_1","volume-title":"Anatomical Techniques","author":"Tompsett D. H.","year":"1970"},{"key":"e_1_2_10_51_1","volume-title":"Sheep as animal model in minimally invasive neurosurgery in EDEN2020. Ph.D. Thesis","author":"Trovatelli M.","year":"2020"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcs.2257"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.10.029"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awq203"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2014.12.002"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci10040211"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2004.04.034"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2013.01.006"}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.12983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T11:45:48Z","timestamp":1705923948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ahe.12983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,11]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/ahe.12983"],"URL":"https:\/\/doi.org\/10.1111\/ahe.12983","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"value":"0340-2096","type":"print"},{"value":"1439-0264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,11]]},"assertion":[{"value":"2023-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12983"}}