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Six male adult felines' tongue tissue samples consist of the study's material. Samples were fixed in 10% formaldehyde. MC number and distribution in the feline tongue were assessed using toluidine blue. Also, sections taken from blocks were stained in alcian blue\/safranin O (AB\/SO) combined dyes to determine the MC subtypes. The Streptavidin biotin complex method using anti\u2010chymase and anti\u2010tryptase primary antibodies was used for immunohistochemistry. Metachromatic MCs were mainly observed in the lamina propria close to the multilayered keratinized stratified squamous epithelium. The high number of MCs in this region may be because the dorsal surface of the tongue plays an essential role in the defence system of tongue tissue and, thus, of the body as a whole. Additionally, the number of MCs stained with AB (+) (1.7\u2009\u00b1\u20090.08) in the feline tongue was statistically higher than those with SO (+) (0.18\u2009\u00b1\u20090.02). This might be interpreted as an indication that MC heterogeneity may be due not only to their staining properties but also to their localization. It is also conceivable that the high histamine content may be a factor in this. Tryptase\u2010positive MCs were found in the loose connective tissue around blood vessels, between the glands, as solitary cells, or in groups of several cells. Chymase\u2010positive MCs were observed more individually rather than in groups. Moreover, chymase\u2010positive MCs were detected to be located in the filiform papillae subepithelial and in the blood vessels' immediate vicinity. Animals often lick themselves to clean themselves and promote healing. For this reason, it is very important to protect the tongue, which is in direct contact with the external environment, against foreign agents. Considering both the functional and protective properties of the tongue, we concluded that MCs may play a role in oral cavity immunity and protective effect.<\/jats:p>","DOI":"10.1111\/ahe.13069","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T03:22:33Z","timestamp":1717471353000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Histochemical and immunohistochemical investigation of the number and localization of mast cells in the feline tongue"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9310-1200","authenticated-orcid":false,"given":"Tu\u011frul","family":"Ertu\u011frul","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine Ondokuz Mayis University  Samsun 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