{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:59:17Z","timestamp":1777517957437,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004214","name":"Pamukkale \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004214","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study investigated the effects of transplanted testicular stromal stem cells (tSSCs) on surgically damaged testis tissue. Ten\u2010week\u2010old male Wistar albino rats were divided into three groups: control (<jats:italic>n<\/jats:italic>\u2009=\u20096), damage (DG) (<jats:italic>n<\/jats:italic>\u2009=\u20096) and testicular stromal stem cell (TSSC) (<jats:italic>n<\/jats:italic>\u2009=\u20096) groups. Surgically induced damage was inflicted on the left testes of both the DG and TSSC groups, with no intervention on the right testes. In the TSSC group, damaged testes were treated with transplanted tSSCs, followed by orchiectomy after 15\u2009days. Testes tissues were stained with haematoxylin\u2013eosin (H&amp;E), and recovery rates of functional structures were assessed by modified Johnsen scoring. The effects of tSSCs on testicular tissue were demonstrated by immunohistochemistry using BAX, BCL\u20102 and caspase 3. Serum testosterone levels were analysed using the enzyme\u2010linked immunosorbent assay (ELISA) method. Surgical damage caused germ cell degeneration in some seminiferous tubules and a decrease in interstitial areas. With tSSC treatment, improvements in testicular architecture were identified through spermatogenesis in the seminiferous tubules and normal histological structures in the interstitial areas. Correspondingly, in the modified Johnsen score, the DG group showed a significant difference compared to the other groups (<jats:italic>p<\/jats:italic>\u2009=\u20090.001). High expressions of BAX, BCL\u20102 and caspase\u20103 in the DG group revealed prominent features of apoptosis. With the injection of tSSCs, these expressions significantly normalized according to H score analysis (all <jats:italic>p<\/jats:italic>\u2009=\u20090.004). Although serum testosterone levels in the tSSC group were higher compared to the control and DG groups, this difference was not statistically significant (<jats:italic>p<\/jats:italic>\u2009=\u20090.119). This study suggests transplanting tSSCs could accelerate tissue healing after testicular sperm extraction (TESE) surgery for azoospermia patients, potentially paving the way for a new and important clinical treatment.<\/jats:p>","DOI":"10.1111\/ahe.13100","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T17:06:00Z","timestamp":1723568760000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The effects of testicular stromal stem cells on surgically injured testicular tissue in rats"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6816-1367","authenticated-orcid":false,"given":"Faruk","family":"Altinbasak","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Hitit University  Corum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1992-7909","authenticated-orcid":false,"given":"Murat Serkant","family":"Unal","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5609-9594","authenticated-orcid":false,"given":"Semih","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Ordu University  Ordu Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9099-515X","authenticated-orcid":false,"given":"Gul","family":"Yildirim","sequence":"additional","affiliation":[{"name":"Department of Stem Cell, Faculty of Medicine Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/6500587"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13760"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.21608\/ijma.2022.237915"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2016.0022"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/529589"},{"issue":"1","key":"e_1_2_9_7_1","first-page":"9","article-title":"Medical treatment of male infertility","volume":"3","author":"Dabaja A. 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