{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:48:34Z","timestamp":1785422914816,"version":"3.56.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,12]],"date-time":"2024-10-12T00:00:00Z","timestamp":1728691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["7621"],"award-info":[{"award-number":["7621"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In vitro maturation (IVM) of immature oocytes is a valuable method to enhance the rate of mature oocytes available for fertilisation. In the current study, platelet\u2010rich plasma (PRP) was employed in IVM medium of immature oocytes. Harvested germinal vesicle stage oocytes with cumulus cells from female mature BALB\/c mice divided into two groups of control and experiment. In the experimental group, GV oocytes matured in the IVM medium supplemented with 5% PRP, while in the control group, GV oocytes matured in the IVM medium without PRP. The percentage of GV, MI, MII and degenerated oocytes, zona pellucida thickness, perivitelline space size, diameter of mature oocytes, gene expression of apoptosis\u2010related factors and subsequent development of matured oocytes were assessed. The PRP group displayed significantly improved outcomes in various parameters, including a higher proportion of MII and fertilised oocytes, cleavage and blastocyst embryos, compared to the control group. Moreover, the thickness of the zona pellucida was significantly lower in the PRP group than in the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Furthermore, the PRP group demonstrated a significant decrease in the expression of transcripts associated with apoptosis (<jats:italic>Bax<\/jats:italic> and <jats:italic>caspase\u20103<\/jats:italic>); however, in the PRP group, a substantial increase in the expression of Bcl2l1, an apoptosis inhibitor, was observed when compared to the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In conclusion, addition of PRP to the IVM culture media significantly increased oocyte maturation rate, leading to improved fertilisation and subsequent embryonic development. This enhancement highlights the positive influence of PRP on overall in\u00a0vitro maturation efficiency and early embryonic stages.<\/jats:p>","DOI":"10.1111\/ahe.13112","type":"journal-article","created":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T05:23:26Z","timestamp":1728797006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>PRP<\/scp> Influences Maturation and Fertilisation of Immature Mouse Oocytes"],"prefix":"10.1111","volume":"53","author":[{"given":"Sanaz","family":"Alaee","sequence":"first","affiliation":[{"name":"Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Stem Cells Technology Research Center Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Infertility Research Centre Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Zal","sequence":"additional","affiliation":[{"name":"Infertility Research Centre Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Biochemistry Department, School of Medicine Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vahid","family":"Razban","sequence":"additional","affiliation":[{"name":"Stem Cells Technology Research Center Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahereh","family":"Talaei\u2010Khozani","sequence":"additional","affiliation":[{"name":"Histomorphometry and Stereology Research Center, Anatomy Department, Medical School Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Tissue Engineering Lab, Anatomy Department, Medical School Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Shokri","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Khodabandeh","sequence":"additional","affiliation":[{"name":"Stem Cells Technology Research Center Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2023.06.011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43043-022-00119-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2021.1968542"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.958"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199415000519"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbre.2022.04.001"},{"issue":"3","key":"e_1_2_9_8_1","first-page":"161","article-title":"Effects of Dextran\u2010Coated Superparamagnetic Iron Oxide Nanoparticles on Mouse Embryo Development, Antioxidant Enzymes and Apoptosis Genes Expression, and Ultrastructure of Sperm, Oocytes and Granulosa Cells","volume":"14","author":"Bakhtari A.","year":"2020","journal-title":"International Journal of Fertility & Sterility"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-022-00895-5"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.14219"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11060833"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-021-02263-5"},{"issue":"3","key":"e_1_2_9_13_1","first-page":"123","article-title":"Assessment of Fertility, Progesterone Profiles and Oxidative Stress Parameters After Estrus Induction and Artificial Insemination in Cows","volume":"9","author":"Dinga H. F.","year":"2021","journal-title":"Journal of Infertility and Reproductive Biology"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2023.02.010"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.4274\/tjod.23911"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/02772248.2018.1485923"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199411000554"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.18-0043"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-022114-110822"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.04.008"},{"issue":"1","key":"e_1_2_9_21_1","first-page":"10","article-title":"Effects of Anethum Graveolens L. (Dill) on Oocyte and Fertility of Adult Female Rats","volume":"16","author":"Monsefi M.","year":"2015","journal-title":"Journal of Reproduction & Infertility"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977194.2021.1900168"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/5702970"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23420"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mefs.2015.01.006"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12522-015-0220-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.623852"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejtm.2022.10409"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60784-5"},{"issue":"2","key":"e_1_2_9_30_1","first-page":"74","article-title":"The Effects of Progesterone on Oocyte Maturation and Embryo Development","volume":"7","author":"Salehnia M.","year":"2013","journal-title":"International Journal of Fertility & Sterility"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/532519"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.03.003"},{"issue":"1","key":"e_1_2_9_33_1","first-page":"260","article-title":"Correlation Between Morphological Abnormalities in the Human Oocyte Zona Pellucida, Fertilization Failure and Embryonic Development","volume":"9","author":"Shi S.\u2010L.","year":"2016","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-022-00906-5"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-022-10039-z"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD19131"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20764"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/09603271211048184"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1797-5"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00186-5"}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.13112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T12:01:28Z","timestamp":1731585688000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ahe.13112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,12]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1111\/ahe.13112"],"URL":"https:\/\/doi.org\/10.1111\/ahe.13112","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"value":"0340-2096","type":"print"},{"value":"1439-0264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,12]]},"assertion":[{"value":"2024-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13112"}}