{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:20:03Z","timestamp":1778224803773,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T00:00:00Z","timestamp":1741478400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to determine the embryotoxic and teratogenic effects of doxycycline on chicken embryos. Eggs placed in the incubator were checked with an ovoscope on the 7th day, and a total of 210 fertile chicken eggs were randomly divided into 7 groups. Doxycycline was applied to the experimental groups at 2500, 1250, 625, 312, and 156\u2009\u03bcg\/50\u2009\u03bcL volumes, respectively. Group 6 was evaluated as the Negative Control Group. This group was placed in the incubator again without any treatment. Group 7 was administered 50\u2009\u03bcL of 0.9% NaCl solution used as the solvent of the drug. The number of live and dead embryos was determined after the completion of incubation. Mortality was observed depending on the dose, and death was determined in all chicks at the highest dose (2500\u2009\u03bcg\/50\u2009\u03bcL,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). No macroscopic anomalies were observed. While a decrease in mortality rates was observed depending on the dose (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), the mortality rate in the 156\u2009\u03bcg\/50\u2009\u03bcL dose (Group 5) was similar to the Positive and Negative control groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Femur and tibia parameters could not be measured because all embryos in Group 1 (2500\u2009\u03bcg\/50\u2009\u03bcL) died. The length and thickness of the right and left femur and tibia in Group 2 (1250\u2009\u03bcg\/50\u2009\u03bcL) were statistically significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) lower than those of the other groups. As a result, it can be stated that doxycycline use should be avoided in pregnant mammals, especially during the development phase of organs, unless necessary.\n                  <\/jats:p>","DOI":"10.1111\/ahe.70030","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:12:55Z","timestamp":1741752775000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Determination of Embryotoxic Dose Limit of Doxycycline and Its Effects on Some Long Bones by In Ovo Model"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4079-500X","authenticated-orcid":false,"given":"Mustafa Sedat","family":"Arslan","sequence":"first","affiliation":[{"name":"Department of Anatomy, Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selvinaz","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"\u0130\u015fgalaman Mah  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reyhan Rabia","family":"K\u00f6k","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamil","family":"Be\u015foluk","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Orhun","family":"Dayan","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22613"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(60)90011-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2018.09.008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.15312\/EurasianJVetSci.2021.328"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2022.05.016"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029\u20107844(97)00005\u20107"},{"key":"e_1_2_9_8_1","unstructured":"Food and Drug Administration.2023a.\u201cDoxycycline Use by Pregnant and Lactating Women.\u201dhttps:\/\/www.fda.gov\/drugs\/bioterrorism\u2010and\u2010drug\u2010preparedness\/doxycycline\u2010use\u2010pregnant\u2010and\u2010lactating\u2010women."},{"key":"e_1_2_9_9_1","unstructured":"Food and Drug Administration.2023b.\u201cDoxycycline Capsules.\u201dhttps:\/\/www.accessdata.fda.gov\/drugsatfda_docs\/label\/2022\/050641Orig1s032lbl.pdf."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/10915818409104398"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1520\u20106866(1990)2:3\/4<255::AID\u2010TCM1770020307>3.0.CO;2\u2010M"},{"key":"e_1_2_9_12_1","first-page":"588","article-title":"Chick Embryotoxicity Screening Test\u2014130 Substances Tested","volume":"23","author":"Jelinek R.","year":"1985","journal-title":"Indian Journal of Experimental Biology"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2016.02.009"},{"key":"e_1_2_9_14_1","first-page":"13","article-title":"The Use of Chick Embryos in Embryotoxicity and Teratogenicity Tests","volume":"40","author":"Ozparlak H.","year":"2015","journal-title":"SUFEFD"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahe.13101"},{"key":"e_1_2_9_16_1","first-page":"214","article-title":"Teratogenicity in Dogs and Cats \u2010 A Review for Practitioners and Toxicologists","volume":"94","author":"Pichler L.","year":"2007","journal-title":"Veterinary Medicine in Australia"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2018.1535593"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656561003733599"},{"issue":"8","key":"e_1_2_9_19_1","first-page":"2390","article-title":"The Effect of Doxycycline on the Viability of the Quail Embryo During Incubation Period","volume":"2","author":"Tavakkoli H.","year":"2014","journal-title":"International Journal of Advanced Biological and Biomedical 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