{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:17:08Z","timestamp":1753877828922,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T00:00:00Z","timestamp":1745020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN 05364\u20102019"],"award-info":[{"award-number":["RGPIN 05364\u20102019"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>There are two main subforms of the teleost fish Mexican tetra (<jats:styled-content style=\"fixed-case\"><jats:italic>Astyanax mexicanus<\/jats:italic><\/jats:styled-content>): the river\u2010dwelling surface fish and the cave\u2010dwelling blind cavefish. Recently, this species has become a popular animal model in developmental biology research. It was reported that cavefish bore more teeth than surface fish; however, a detailed description of oral dentition development and replacement in the Mexican tetra remains unavailable. In this study, we investigated the differences in tooth count between surface fish and cavefish by characterising the features of mandibular dentition in adult Mexican tetras and explored the process of first\u2010generation dentition development in larval fish. Regarding early dentition development, larval surface fish and Pach\u00f3n cavefish at 12\u2010, 18\u2010, 21\u2010, 24\u2010, and 30\u2010days post\u2010fertilisation were euthanised. Haematoxylin and eosin staining was performed for histological analysis, and immunohistochemistry was employed to detect the expression of the odontogenic marker Sox2. The results show that Mexican tetras replaced functional teeth via the successional dental lamina marked by Sox2 expression. Adult Pach\u00f3n cavefish possessed more intraosseous replacement teeth. Mandibular tooth development in larval fish was also marked by Sox2 expression, and no evidence supported tooth development in a continual dental lamina. Our findings describe the appearance of a successional dental lamina in tooth replacement in the Mexican tetra but provide no evidence regarding a continual dental lamina. In the Mexican tetra, teeth of the first\u2010generation dentition may develop independently. The results of this project will offer valuable histological information for future research into tooth development in the Mexican tetra.<\/jats:p>","DOI":"10.1111\/ahe.70038","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T14:04:25Z","timestamp":1747317865000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploration of Dentition Development and Replacement in Two Forms of Mexican Tetra (<scp><i>Astyanax mexicanus<\/i><\/scp>)"],"prefix":"10.1111","volume":"54","author":[{"given":"Zheng","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Rady Faculty of Health Sciences Dr Gerald Niznick College of Dentistry, University of Manitoba  Winnipeg Manitoba Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0279-1857","authenticated-orcid":false,"given":"Devi","family":"Atukorallaya","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Rady Faculty of Health Sciences Dr Gerald Niznick College of Dentistry, University of Manitoba  Winnipeg Manitoba Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2014.09.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.07.008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/2041-9139-4-28"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-12-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242015061"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.013672"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolinnean\/bly082"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.052098ah"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2007.00209.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.13045"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21275"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0121"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2013.11.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517720158"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.089599"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12729"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/jdb9010008"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00445"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-012023-014003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037685"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.2005.00193.x"},{"key":"e_1_2_9_24_1","unstructured":"Woodard N.2020. \u201cAre Florida Manatees (Trichechus Manatus Latirostris) Wearing Their Teeth Beyond Functionality? Interspecific and Intraspecific Mesowear in Manatees.\u201d Honors theses [Internet].https:\/\/digitalcommons.andrews.edu\/honors\/233."}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ahe.70038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T13:41:36Z","timestamp":1748353296000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ahe.70038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,19]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/ahe.70038"],"URL":"https:\/\/doi.org\/10.1111\/ahe.70038","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"type":"print","value":"0340-2096"},{"type":"electronic","value":"1439-0264"}],"subject":[],"published":{"date-parts":[[2025,4,19]]},"assertion":[{"value":"2025-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70038"}}