{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:08:41Z","timestamp":1791349721316,"version":"4.3.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The testes of mammals are responsible for gamete production and the secretion of the sex hormone testosterone. Within the seminiferous tubules, spermatogenesis occurs, where germline cells undergo division and differentiation until the formation of spermatozoa. Meanwhile, in the intertubular compartment, Leydig cells produce and secrete testosterone, a hormone essential for spermatogenesis. <jats:styled-content style=\"fixed-case\"><jats:italic>Monodelphis americana<\/jats:italic><\/jats:styled-content> is a marsupial species endemic to Brazil, commonly known as the three\u2010striped short\u2010tailed opossum. The reproductive biology of marsupials remains relatively unexplored despite being a crucial tool for understanding species\u2010specific reproductive strategies, which can aid in management and conservation efforts. In this study, <jats:styled-content style=\"fixed-case\"><jats:italic>M. americana<\/jats:italic><\/jats:styled-content> exhibited a body weight of 36.7\u2009g, with a gonadosomatic index (GSI) of 0.47%, a tubular somatic index (TSI) of 0.36% and an interstitial somatic index (ISI) of 0.27%. The proportion of seminiferous tubules was 83.16%, indicating a significant investment in sperm production. The mitotic and meiotic indices were 1.87 and 2.64 cells per division, respectively, with an overall spermatogenic yield of 12.68. The intertubular compartment accounted for 16.84% of the testicular parenchyma, of which 13.13% consisted of Leydig cells, with a total count of 22.75\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> Leydig cells per testis. The findings suggest that <jats:styled-content style=\"fixed-case\"><jats:italic>M. americana<\/jats:italic><\/jats:styled-content> prioritises spermatogenic parameters over the intertubular compartment, implying that males exhibit a promiscuous reproductive behaviour.<\/jats:p>","DOI":"10.1111\/ahe.70047","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T09:53:07Z","timestamp":1748425987000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Testicular Histomorphometric Parameters and Spermatogenic Dynamics of <scp><i>Monodelphis americana<\/i><\/scp> (M\u00fcller, 1776) (Didelphimorphia: Didelphidae)"],"prefix":"10.1111","volume":"54","author":[{"given":"Ana Luiza Pereira","family":"Martins","sequence":"first","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6530-4684","authenticated-orcid":false,"given":"Fernanda Carolina Ribeiro","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Structural Biology Federal University of Tri\u00e2ngulo Mineiro  Uberaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grasielle Avelar Vieira","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Lopes","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriele Ribeiro","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Department of Animal Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gleide Fernandes","family":"de Avelar","sequence":"additional","affiliation":[{"name":"Department of Morphology Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Luis Pinto","family":"da Matta","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001100106"},{"key":"e_1_2_9_3_1","first-page":"167","volume-title":"Animais de Laborat\u00f3rio: Cria\u00e7\u00e3o e Experimenta\u00e7\u00e3o","author":"Andrade A.","year":"2002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1977.445818x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-46702009000100011"},{"key":"e_1_2_9_6_1","volume-title":"Os Marsupiais do Brasil","author":"C\u00e1ceres N.","year":"2013"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.109.009787"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.07.016"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113182"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.12.047"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116680"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/9.5.500"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahe.12642"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.07.004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.014"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12165"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7208\/chicago\/9780226282428.001.0001"},{"key":"e_1_2_9_18_1","first-page":"255","volume-title":"Molecular Mechanisms in Spermatogenesis","author":"Ge R.","year":"2008"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod25.1.217"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.7882\/AZ.2010.020"},{"key":"e_1_2_9_21_1","first-page":"137","article-title":"A Formaldehyde\u2010Glutaraldehyde Fixative of High Osmolaty for Use in Electron Microscopy","volume":"27","author":"Karnovsky M. 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