{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T01:58:55Z","timestamp":1755223135003,"version":"3.43.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010324","name":"Secretar\u00eda General de Ciencia y T\u00e9cnica, Universidad Nacional del Nordeste","doi-asserted-by":"publisher","award":["PI 22B005","PI No 145"],"award-info":[{"award-number":["PI 22B005","PI No 145"]}],"id":[{"id":"10.13039\/501100010324","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The order Chiroptera is one of the most diverse orders in terms of the number of species; however, very few studies have been conducted in this group regarding its embryonic development. In this area, studies have focused on staging through morphological characters, but those describing organogenesis are scarce. Therefore, this work describes the gonadogenesis of <jats:styled-content style=\"fixed-case\"><jats:italic>Eumops patagonicus<\/jats:italic><\/jats:styled-content>, a Sudamerican insectivorous bat, with an emphasis on ovarian development and determination of the migration stage of the primordial germ cells (PGCs) through immunostaining. Pregnant females of this species were captured to carry out the study, and the embryos were processed to obtain histological sections using the conventional histology technique and immunohistochemistry (OCT4, PCNA, Bax and Bcl\u2010XL). Critical stages of gonadal differentiation were described, starting from the formation of the urogenital ridge and the identification of PGCs with the positive reaction for OCT4 in embryological development stage 13. At stage 17 of development, an undifferentiated gonad with active proliferation is observed and then a defined testis (S. 21) and ovaries (S.23 and S.25). The embryo ovaries S. 23 show a cortex formed for ovogonia cysts\/nets that have a craniocaudal development with differences in the immunolabelling of PCNA, Bax and Bcl\u2010XL. The embryo ovaries S. 25 show the primordial follicles in the ovary cortex. The results determined that there is a conserved pattern in embryonic development in this order when comparing <jats:styled-content style=\"fixed-case\"><jats:italic>E. patagonicus<\/jats:italic><\/jats:styled-content> with frugivorous bats. This information is important for the establishment of relations in development in mammals and within the order Chiroptera.<\/jats:p>","DOI":"10.1111\/ahe.70049","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T11:06:46Z","timestamp":1749726406000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Histological Description of Gonadal Development in a Neotropical Insectivorous Bat <scp><i>Eumops patagonicus<\/i><\/scp> (Chiroptera: Molossidae)"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9967-8393","authenticated-orcid":false,"given":"Florencia Evelyn","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaciones Bioqu\u00edmicas (LIBIM), Facultad de Medicina, Instituto de Qu\u00edmica B\u00e1sica y Aplicada del NEA, (IQUIBA NEA\u2010 UNNE\u2010 CONICET) Universidad Nacional del Nordeste  Corrientes Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Beatriz","family":"Olea","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad Nacional del Nordeste  Corrientes Argentina"},{"name":"Facultad de Ciencias Veterinarias, Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad de Buenos Aires  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Aguirre","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaciones Bioqu\u00edmicas (LIBIM), Facultad de Medicina, Instituto de Qu\u00edmica B\u00e1sica y Aplicada del NEA, (IQUIBA NEA\u2010 UNNE\u2010 CONICET) Universidad Nacional del Nordeste  Corrientes Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Marcelo","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"},{"name":"Facultad de Ciencias Veterinarias, Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad de Buenos Aires  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD12126"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.09.110193.002455"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0578"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.117.1.273"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.042308.13350"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.9.851"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/6.11.999"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2022.07.001"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ando.2020.04.004"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20780"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2022.03.002"},{"issue":"9","key":"e_1_2_11_13_1","first-page":"2244","article-title":"POU5F1 (OCT3\/4) Identifies Cells With Pluripotent Potential in Human Germ Cell Tumors","volume":"63","author":"Looijenga L. H.","year":"2003","journal-title":"Cancer Research"},{"volume-title":"Introduction to Embryonic Development","year":"2004","author":"Oppenheimer S. B.","key":"e_1_2_11_14_1"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006101078"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2017.08.002"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23896"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmammal\/gyw078"},{"key":"e_1_2_11_19_1","first-page":"147","article-title":"Primordial Germ Cells Migration: Morphological and Molecular Aspects","volume":"2","author":"Soto\u2010Suazo M.","year":"2018","journal-title":"Animal Reproduction"},{"volume-title":"AVMA Guidelines for the Euthanasia of Animals","year":"2013","author":"Underwood W.","key":"e_1_2_11_20_1"},{"volume-title":"Ecological and Behavioral Methods for the Study of Bats","year":"2009","author":"Wilkinson G. 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