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The objective of the present study was to establish normal quantitative parameters for the cerebellum during intrauterine development in sheep, using unbiased design\u2010based stereological methods. Twenty sheep embryos were divided into four gestational age groups (9\u201311, 12\u201314, 15\u201317 and 18\u201320\u2009weeks of gestation). The orientator method was used to obtain isotropic, uniform and random sections. The fractional volume of white matter, grey matter and different layers was estimated by a point\u2010counting system. The cerebellar surface area was estimated through the use of test lines. The total volume of the cerebellum increased from 0.1142\u2009\u00b1\u20090.02644\u2009cm<jats:sup>3<\/jats:sup> to 1.4717\u2009\u00b1\u20090.3558\u2009cm<jats:sup>3<\/jats:sup> during development. The volume of white matter increased from 0.0785\u2009\u00b1\u20090.02616\u2009cm<jats:sup>3<\/jats:sup> to 0.5316\u2009\u00b1\u20090.12677\u2009cm<jats:sup>3<\/jats:sup>, and the volume of grey matter showed an increase from 0.0785\u2009\u00b1\u20090.02616\u2009cm<jats:sup>3<\/jats:sup> to 0.5316\u2009\u00b1\u20090.12677\u2009cm<jats:sup>3<\/jats:sup>. The volume of the external granular, molecular and internal granular layers increased from 0.009\u2009\u00b1\u20090.00249\u2009cm<jats:sup>3<\/jats:sup>, 0.0078\u2009\u00b1\u20090.00175\u2009cm<jats:sup>3<\/jats:sup>, 0.0189\u2009\u00b1\u20090.00403\u2009cm<jats:sup>3<\/jats:sup> to 0.0942\u2009\u00b1\u20090.01986\u2009cm<jats:sup>3<\/jats:sup>, 0.2311\u2009\u00b1\u20090.04582\u2009cm<jats:sup>3<\/jats:sup>, 0.5367\u2009\u00b1\u20090.1666\u2009cm<jats:sup>3<\/jats:sup>, respectively. The surface area of the cerebellum increased from 5.54\u2009\u00b1\u20091.75\u2009cm<jats:sup>2<\/jats:sup> to 53\u2009\u00b1\u200911.6\u2009cm<jats:sup>2<\/jats:sup>. All aforementioned parameters showed a positive significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) correlation with gestational age. The standard data of this research could be used as a reference for the accurate surveying of cerebellar development and also for practical applications in veterinary embryological neurology.<\/jats:p>","DOI":"10.1111\/ahe.70064","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T11:37:27Z","timestamp":1757677047000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Three\u2010Dimensional Morphometry of the Cerebellum During Intrauterine Development in Sheep Using Unbiased Design\u2010Based Stereology"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0735-8788","authenticated-orcid":false,"given":"Javad","family":"Sadeghinezhad","sequence":"first","affiliation":[{"name":"Department of Basic Sciences, Faculty of Veterinary Medicine University of Tehran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fakhra","family":"Borgheie","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Faculty of Veterinary Medicine University of Tehran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4343-4453","authenticated-orcid":false,"given":"Omid","family":"Zehtabvar","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Faculty of Veterinary Medicine University of Tehran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Bayat","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Faculty of Veterinary Medicine University of Tehran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/2141098a0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903260405"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10884"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.06.013"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dev.20563"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458508101934"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.08.056"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2008.12.003"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623310385140"},{"volume-title":"Handbook of Physiology, Section\u00a01: The Nervous System, Volume II Motor Control, Part 2","year":"1981","author":"Brooks V.","key":"e_1_2_7_11_1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-105"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-5629(00)00225-8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2006.07.019"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2013.03.028"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006123-199710000-00029"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ntt.2008.03.064"},{"key":"e_1_2_7_18_1","first-page":"253","article-title":"The Developing Brain","volume":"4","author":"Davison A. 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