{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:44:09Z","timestamp":1782711849645,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The greater rhea (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Rhea americana<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), the largest bird species in South America, has significant economic value due to its meat, eggs, leather, feathers and oil. However, its lack of external sexual dimorphism poses challenges for reproductive management and breeding programmes. Key factors involved in avian gonadal differentiation include the cytochrome P450c17 (CYP17A1), P450 aromatase and the oestrogen receptor alpha (ER\u03b1). This study investigated the spatiotemporal immunolocalization of these proteins in germ and somatic cells of gonadal crests and developing gonads in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. americana<\/jats:italic>\n                    <\/jats:styled-content>\n                    embryos from Day 9 to Day 24 of incubation. Immunohistochemistry revealed consistent expression of all three markers throughout gonadogenesis, with distinct intensity patterns between sexes and cell types. P450c17 has been detected in both germ and somatic cells in testes and ovaries, with increasing expression from the onset of sexual differentiation. Aromatase and ER\u03b1 were strongly expressed in the ovarian cortex and lacunar regions, and moderately present in testicular cords and interstitium. These findings suggest coordinated endocrine signalling between germ and somatic cells during embryonic sexual differentiation in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. americana<\/jats:italic>\n                    <\/jats:styled-content>\n                    and provide foundational insight for future research on reproductive development and sex control in ratites.\n                  <\/jats:p>","DOI":"10.1111\/ahe.70091","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T07:59:22Z","timestamp":1771055962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immunostaining of P450c17, Aromatase and Oestrogen Receptor Alpha in Germ and Somatic Cells During Gonadal Development in Greater Rhea"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7410-5057","authenticated-orcid":false,"given":"Maril\u00fa","family":"Cristofoli","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Cristina Calado","family":"de Brito","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayla Magalh\u00e3es de Oliveira","family":"Alcoba\u00e7a","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia Maria Pia Montenegro","family":"Donoso","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Escuela de Medicina Veterinaria, Unidad de Anatom\u00eda Veterinaria, Facultad de Recursos Naturales y Medicina Veterinaria Universidad Santo Tom\u00e1s  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9880-1142","authenticated-orcid":false,"given":"Fabiana Morse Gosson","family":"Jorge","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Aur\u00e9lio Schiavo","family":"Novaes","sequence":"additional","affiliation":[{"name":"University of International Integration of Afro\u2010Brazilian Lusophony \u2013 UNILAB  Reden\u00e7\u00e3o 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