{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:19:20Z","timestamp":1787239160829,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Blastocyst biopsy allows genomic selection of pre\u2010implantation bovine embryos, reducing generation interval and enhancing the genetic gain. We aimed to evaluate the suitability of a simple biopsy procedure, performed by hands, using a splitting microblade and a stereoscope, on in\u00a0vitro\u2010produced embryos. Crossbred\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bos taurus<\/jats:italic>\n                    <\/jats:styled-content>\n                    x\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. indicus<\/jats:italic>\n                    <\/jats:styled-content>\n                    blastocysts at Day 6 or 7 after in\u00a0vitro fertilization (IVF) were biopsied and cultured in\u00a0vitro for 48\u2009h. Biopsy reduced (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) embryo development as noted by the lower blastocoel formation\/re\u2010expansion rates in both Day 6 (33.3% vs. 71.4%) and Day 7 (66.6% vs. 89.8%) blastocysts, when compared to non\u2010biopsied embryos. Biopsy on Day 7 decreased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) cell number (112.8\u2009\u00b1\u20096.3 vs. 149.9\u2009\u00b1\u20095.6) and increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) apoptotic index (14.9\u2009\u00b1\u20091.4 vs. 6.2\u2009\u00b1\u20091.2). In a second experiment, the IVF blastocysts were biopsied in the farm, and cultured for 3\u2009h. Re\u2010expanded blastocysts, corresponding to 78.3% biopsied embryos, were transferred to synchronized recipients. Pregnancy (57.4% vs. 62%), delivery (51.0% vs. 48%), gestation length (285.5\u2009\u00b1\u20092.0 vs. 284.6\u2009\u00b1\u20091.2\u2009days) and birthweight (30.5\u2009\u00b1\u20091.8 vs. 29.9\u2009\u00b1\u20091.2\u2009kg) were similar (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05) between handmade biopsy and non\u2010biopsied embryos. Whole genome amplification of biopsied samples resulted in 256.0\u2009\u00b1\u2009155.9\u2009ng\/\u03bcL DNA. The mean call rate was 75.3%\u2009\u00b1\u20094.3% and the imputed GEBV for 305\u2010day milk yield was 839.21\u2009\u00b1\u200961.6\u2009kg. In conclusion, handmade biopsy can be performed at small laboratories and at farm level, contributing to reduce costs for embryo genomic selection. Pregnancy, calf delivery and birthweight were not compromised, and samples are suitable for downstream applications such as genotyping.\n                  <\/jats:p>","DOI":"10.1111\/ahe.70128","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T14:32:28Z","timestamp":1778596348000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Handmade Biopsy for Genotyping of In\u00a0Vitro\u2010Produced Bovine Embryos"],"prefix":"10.1111","volume":"55","author":[{"given":"Luiz Sergio Almeida","family":"Camargo","sequence":"first","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Capobiango Romano","family":"Quint\u00e3o","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Medeiros Cruz Lima","family":"de Martins","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"},{"name":"Fluminense Federal University  Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Vinicius Gualberto Barbosa","family":"Silva","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agostinho Jorge Reis","family":"dos Camargo","sequence":"additional","affiliation":[{"name":"Agriculture Research Company of the Rio de Janeiro State (PESAGRO\u2010RIO)  Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celio","family":"Freitas","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele Ribeiro Lima","family":"de Reis","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Antonio","family":"Machado","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Slade","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle  Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034130"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016\u201011811"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/541384"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2012\u20106208"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.11.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/214074"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1519061113"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2527\/1998.76suppl_352x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2012.09.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(01)00641\u20100"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22230"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0980467"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/157.4.1819"},{"key":"e_1_2_9_15_1","unstructured":"Misztal I. 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