{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T11:12:43Z","timestamp":1784200363756,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Anatomical preservation is essential for teaching and research; however, conventional formaldehyde\u2010based techniques present limitations due to toxicity, volatility and environmental impact. Plastination provides excellent morphological quality, but its high cost, reliance on flammable solvents and requirement for specialized equipment limit its accessibility. This study proposes a low\u2010cost alternative technique for the preservation of hearts and lungs using internal application of poly(vinyl acetate) (PVAc) followed by air drying. Organs from two canines, one ovine and one lagomorph were injected with PVAc, fixed in 3.7% formaldehyde for 72\u2009h, and subsequently dehydrated at room temperature for approximately 30\u2009days. The resulting specimens maintained their three\u2010dimensional conformation, exhibited reduced weight, absence of odour and good resistance to handling, and allowed subsequent painting for didactic purposes. Despite some tissue darkening, overall morphological integrity was preserved, producing specimens suitable for anatomical demonstration. This technique represents a simple, reproducible and cost\u2010effective alternative to traditional plastination, significantly reducing formaldehyde exposure and eliminating the need for flammable solvents or complex laboratory infrastructure.<\/jats:p>","DOI":"10.1111\/ahe.70137","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:37:08Z","timestamp":1779881828000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Low\u2010Cost Technique for Three\u2010Dimensional Cardiopulmonary Preservation Using Internal Poly(Vinyl Acetate) (\n                    <scp>PVAc<\/scp>\n                    ) Injection"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5195-3202","authenticated-orcid":false,"given":"Fabio Cesar Magioli","family":"Abdala","sequence":"first","affiliation":[{"name":"Department of Surgery, Anatomy Division, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo (FMVZ\/USP)  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9981-1230","authenticated-orcid":false,"given":"Silvio Pires","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Surgery, Anatomy Division, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo (FMVZ\/USP)  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1143-4428","authenticated-orcid":false,"given":"Silmara Santos","family":"Lazzaretti","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3885-0732","authenticated-orcid":false,"given":"Maria Clara Ucker","family":"Cost\u00f3dio","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0837-9612","authenticated-orcid":false,"given":"Bruno Cesar","family":"Schimming","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8744-2968","authenticated-orcid":false,"given":"Rinaldo Jos\u00e9","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3663-7575","authenticated-orcid":false,"given":"Vinicius Pedro Silva","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Surgery, Anatomy Division, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo (FMVZ\/USP)  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7870-5404","authenticated-orcid":false,"given":"Selma Maria Michelin","family":"Matheus","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4871-5625","authenticated-orcid":false,"given":"Tais Harumi de Castro","family":"Sasahara","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386454-3.00388-2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07373939108916727"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahe.12486"},{"issue":"7","key":"e_1_2_11_5_1","first-page":"A34","article-title":"Formaldehyde: Evidence of Carcinogenicity","volume":"42","author":"Blackwell M.","year":"1981","journal-title":"American Industrial Hygiene Association Journal"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12160"},{"key":"e_1_2_11_7_1","first-page":"229","article-title":"The Human Cadaver as an Educational Tool: Classification and Comparison With Other Educational Tools","volume":"185","author":"Brenner 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