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However, their postnatal cell\u2010type\u2010specific localization patterns in the testis remain incompletely understood. This study investigated the postnatal cell\u2010type\u2010specific localization patterns of EGFR, EGF, TGF\u2010\u03b1 and AREG in the rat testis using histological and immunohistochemical approaches at four developmental stages. EGFR immunoreactivity was predominantly localized in Sertoli cells, peritubular myoid cells and vascular smooth muscle cells, whereas Leydig cells exhibited weaker immunoreactivity and germ cells lacked staining. In contrast, EGF and AREG exhibited strong and persistent expression in Leydig cells throughout postnatal development. TGF\u2010\u03b1 demonstrated stage\u2010dependent immunoreactivity in Leydig cells and spermatids. Although semiquantitative scores did not reveal significant stage\u2010dependent differences, distinct cell\u2010type\u2010specific localization patterns were observed throughout postnatal development. These findings indicate that EGF family signalling in the postnatal rat testis is primarily mediated through somatic cell populations and support the presence of a paracrine regulatory network involved in the organization of the testicular microenvironment and the regulation of spermatogenesis.<\/jats:p>","DOI":"10.1111\/ahe.70167","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:24:01Z","timestamp":1787059441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Postnatal Cell\u2010Type\u2010Specific Localization of\n                    <scp>EGFR<\/scp>\n                    and Its Ligands (\n                    <scp>EGF<\/scp>\n                    ,\n                    <scp>TGF<\/scp>\n                    \u2010\u03b1 and\n                    <scp>AREG<\/scp>\n                    ) in the Rat Testis"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5996-8225","authenticated-orcid":false,"given":"Yahy Abood Kareem","family":"Alesawi","sequence":"first","affiliation":[{"name":"Graduate School of Health Sciences Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-8230","authenticated-orcid":false,"given":"Emel","family":"Erg\u00fcn","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100264.2005.00651.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.3.680"},{"key":"e_1_2_10_4_1","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft J. 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