{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:11:20Z","timestamp":1789906280179,"version":"4.0.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T00:00:00Z","timestamp":1509062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Human Genetics"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biological sample collection is becoming more common in epidemiology research to obtain DNA for genetic analysis. There are many different DNA collection methods but little evidence on their relative effectiveness. Therefore, we took the opportunity of a prospective case\u2010control study in myeloproliferative neoplasms (MPNs) to compare DNA yield from 8.5 mL PAXgene tubes for whole blood collection versus 2 mL Oragene OG\u2010500 saliva collection kits. MPNs include polycythaemia vera, essential thrombocythaemia, and primary myelofibrosis. These are rare diseases and our exploratory case\u2013control study (MOSAICC) sought to improve knowledge regarding their aetiology and to determine optimal methodology for a larger UK\u2010wide study. Overall, 233 participants were recruited to the MOSIACC study, and we collected 187 blood and 214 saliva samples. The mean DNA yield from blood was 659.18 ng\/\u03bcL, significantly higher than the mean DNA yield from saliva samples (275.79 ng\/\u03bcL). The higher provision of saliva samples might reflect its non\u2010invasive and more convenient nature, compared to blood sample provision. The differences in mean DNA yields might reflect differences in clinical assistance, adherence to instructions, and health status of individuals. In conclusion, both sample collection techniques are simple, effective, and suitable for DNA collection for genetic analysis in future epidemiological research studies but OG\u2010500 kits offer a less invasive alternative for those who refuse to provide blood.<\/jats:p>","DOI":"10.1111\/ahg.12227","type":"journal-article","created":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T07:18:44Z","timestamp":1509088724000},"page":"114-118","source":"Crossref","is-referenced-by-count":9,"title":["The MOSAICC study: Assessing feasibility for biological sample collection in epidemiology studies and comparison of DNA yields from saliva and whole blood samples"],"prefix":"10.1111","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0080-3156","authenticated-orcid":false,"given":"Glen","family":"James","sequence":"first","affiliation":[{"name":"Centre for Public Health Queen's University Belfast  Belfast Northern Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Frances","family":"McMullin","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research and Cell Biology Queen's University Belfast  Belfast Northern Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S","family":"Duncombe","sequence":"additional","affiliation":[{"name":"Department of Haematology University Hospitals Southampton NHS Foundation Trust  Hampshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Clarke","sequence":"additional","affiliation":[{"name":"Centre for Public Health Queen's University Belfast  Belfast Northern Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley A","family":"Anderson","sequence":"additional","affiliation":[{"name":"Centre for Public Health Queen's University Belfast  Belfast Northern Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-8794-5-19"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.22212"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahg.12009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039821"},{"key":"e_1_2_8_7_1","unstructured":"DNA Genotek. (2015).Laboratory protocol for manual purification of DNA from whole saliva sample. Available athttp:\/\/www.dnagenotek.com\/index2.html"},{"key":"e_1_2_8_8_1","unstructured":"Iwasiow R. M. Desbois A. &Birnboim H. C.(2011).Long\u2010term stability of DNA from saliva samples stored in the Oragene\u00ae self\u2010collection kit. Retrieved fromhttp:\/\/dnagenotek.com\/US\/pdf\/PD-WP-005.pdf"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01177.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2288-12-65"},{"key":"e_1_2_8_11_1","volume-title":"Gentra Puregene handbook","author":"Qiagen","year":"2011"},{"key":"e_1_2_8_12_1","unstructured":"Qiagen. (2014).PAXgene blood DNA tubes. 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