{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:35:24Z","timestamp":1771036524705,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T00:00:00Z","timestamp":1510876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["14411960400"],"award-info":[{"award-number":["14411960400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["14DZ1940103"],"award-info":[{"award-number":["14DZ1940103"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Human Genetics"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to investigate the role of the deleted in malignant brain tumors 1 <jats:italic>(DMBT1)<\/jats:italic> gene in the development of nasal polyps, as well as related mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A stable human nasal mucosa epithelial cell (HNEpC) line with low expression of <jats:italic>DMBT1<\/jats:italic> was generated. Three groups were established: a control group (HNEpCs without any treatment), a control short interference RNA (shRNA) group (HNEpCs transfected with an empty vector), and a DMBT1 shRNA group (HNEpCs with silenced <jats:italic>DMBT1<\/jats:italic>). Cell viability, apoptosis, and cell cycle distribution were measured after incubation. Expression of p53, signal transducer and activator of transcription 3 (STAT3), protein kinase B (AKT) and extracellular signal\u2010regulated kinase1\/2 (ERK1\/2) was detected by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the control cell line, HNEpCs with silenced <jats:italic>DMBT1<\/jats:italic> had increased viability and decreased apoptosis. Moreover, after <jats:italic>DMBT1<\/jats:italic> silence, cell numbers were decreased significantly in the G1 phase and increased in the G2 and S phases. <jats:italic>DMBT1<\/jats:italic> silence was associated with increased AKT expression and decreased p53 expression, but it did not alter expression of ERK1\/2 or STAT3 (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). Compared with the control cell line, HNEpCs transfected with an empty vector did not have altered cell viability, apoptosis, cell cycle distribution or expression of AKT, p53, ERK1\/2, or STAT3 (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>DMBT1<\/jats:italic> plays an important role in the growth and division of nasal epithelial cells. The possible mechanism might involve <jats:italic>DMBT1<\/jats:italic> regulating the AKT\u2013p53 pathway to promote cell viability and reduce apoptosis of nasal epithelial cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ahg.12230","type":"journal-article","created":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T11:58:40Z","timestamp":1510919920000},"page":"102-108","source":"Crossref","is-referenced-by-count":10,"title":["Silenced DMBT1 promotes nasal mucosa epithelial cell growth"],"prefix":"10.1111","volume":"82","author":[{"given":"Xiaoqing","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Shanghai Changzheng Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Shanghai Changzheng Hospital  Shanghai 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