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We propose to investigate the shared gene signature between pterygium and MGD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Microarray datasets were retrieved from the Gene Expression Omnibus (GEO) database. Initial processing of the data was performed using the R programming package. Gene\u2010expression values were log2 transformed and normalized by quantile normalization. The differentially expressed genes (DEGs) in each individual dataset were analyzed by the limma package. The integration of different pterygium datasets and gene\u2010expression meta\u2010analysis was conducted by the NetworkAnalyst package. A Venn diagram was created to find the overlapped DEGs between MGD and pterygium datasets. Gene ontology enrichment and pathway analysis were performed using the ToppGene Suite.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found 193 DEGs significantly up\u2010regulated in pterygium, with the combined effect sizes ranging from 1.53 to 3.78. A gene signature consisting of 11 DEGs were found to be shared by pterygium and MGD (<jats:italic>SPRR3<\/jats:italic>, <jats:italic>SERPINB13<\/jats:italic>, <jats:italic>NMU<\/jats:italic>, <jats:italic>KRT10<\/jats:italic>, <jats:italic>IL37<\/jats:italic>, <jats:italic>KRT6B<\/jats:italic>, <jats:italic>PI3<\/jats:italic>, <jats:italic>S100A2<\/jats:italic>, <jats:italic>MAL<\/jats:italic>, <jats:italic>AURKA<\/jats:italic>, and <jats:italic>RGCC<\/jats:italic>), and bioinformatics analyses showed that these overlapped DEGs were significantly enriched in pathways related to keratinization, cell\u2010cycle regulation, and formation of the cornified envelope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We identified a shared gene signature between pterygium and MGD through gene\u2010expression meta\u2010analysis. The analysis of this signature underlined that keratinization\u2010related pathways may play important roles in the development of these two clinically correlated pathologies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ahg.12340","type":"journal-article","created":{"date-parts":[[2019,8,2]],"date-time":"2019-08-02T14:34:00Z","timestamp":1564756440000},"page":"397-404","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Shared gene signature between pterygium and meibomian gland dysfunction uncovered through gene\u2010expression meta\u2010analysis"],"prefix":"10.1111","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5346-7566","authenticated-orcid":false,"given":"Jing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Science, Eye Institute, Eye &amp; ENT Hospital, Shanghai Medical College of Fudan University; NHC Key Laboratory of Myopia (Fudan University) Shanghai Key Laboratory of Visual Impairment and Restoration  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, Eye Institute, Eye &amp; ENT Hospital, Shanghai Medical College of Fudan University; NHC Key Laboratory of Myopia (Fudan University) Shanghai Key Laboratory of Visual Impairment and Restoration  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, Eye Institute, Eye &amp; ENT Hospital, Shanghai Medical College of Fudan University; NHC Key Laboratory of Myopia (Fudan University) Shanghai Key Laboratory of Visual Impairment and Restoration  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqin","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, Eye Institute, Eye &amp; ENT Hospital, Shanghai Medical College of Fudan University; NHC Key Laboratory of Myopia (Fudan University) Shanghai Key Laboratory of Visual Impairment and Restoration  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, Eye Institute, Eye &amp; ENT Hospital, Shanghai Medical College of Fudan University; NHC Key Laboratory of Myopia (Fudan University) Shanghai Key Laboratory of Visual Impairment and Restoration  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp427"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2004.02.002"},{"key":"e_1_2_9_4_1","first-page":"422","article-title":"Abnormal epithelial differentiation and tear film alteration in pterygium","volume":"49","author":"Dong N.","year":"2013","journal-title":"Chinese Journal of Ophthalmology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70139-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1120672118757428"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12606176"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097402"},{"key":"e_1_2_9_9_1","first-page":"936","article-title":"Meibomian gland dysfunction. 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