{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:26:35Z","timestamp":1754396795459},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T00:00:00Z","timestamp":1375660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Asia-Pac J Clncl Oncology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To investigate the contribution of vascular endothelial growth factor (VEGF<\/jats:styled-content>)\u2010D<\/jats:styled-content> to tumor progression, tumor lymphangiogenesis and lymphatic metastasis in epithelial ovarian cancer.<\/jats:p><\/jats:sec>Methods<\/jats:title>The expression profiles of VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> in 18 benign, 14 borderline and 87 malignant epithelial ovarian cancers were examined using immunohistochemical (IHC<\/jats:styled-content>) staining. Lymphatic vessels were identified using IHC<\/jats:styled-content> staining on lymphatic vessel endothelial hyaluronan receptor\u20101 (LYVE<\/jats:styled-content>\u20101), which is a lymph\u2010specific receptor for hyaluronan in identifying lymphatic vessels. The potential correlation among VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content>, lymphatic vessel density (LVD<\/jats:styled-content>) and clinico\u2010pathological factors of the epithelial ovarian cancer was also analyzed.<\/jats:p><\/jats:sec>Results<\/jats:title>Positive IHC<\/jats:styled-content> staining of VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> was observed in 17% of benign, 21% of borderline and 80% of malignant epithelial ovarian tumors specimens. In the epithelial ovarian cancer specimens, the LVD<\/jats:styled-content> was 3.41\u2009\u00b1\u20092.37 in the VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> negative (17 patients), 5.42\u2009\u00b1\u20093.49 in the weak (26 patients), 7.22\u2009\u00b1\u20092.36 in the moderate (27 patients) and 7.35\u2009\u00b1\u20094.06 in the strong (17 patients) groups, respectively. Additionally, the expression of VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> was positively correlated with LVD<\/jats:styled-content> (r\u2009=\u20090.415, P<\/jats:italic>\u2009<\u20090.001). The expression level of VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> was significantly higher in lymph node\u2010positive epithelial ovarian cancer than in lymph node\u2010negative patients (P<\/jats:italic>\u2009=\u20090.009, P<\/jats:italic>\u2009<\u20090.05). The expression of VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> was significantly correlated with lymph node metastasis, I<\/jats:styled-content>nternational F<\/jats:styled-content>ederation of G<\/jats:styled-content>ynecology and O<\/jats:styled-content>bstetrics stage and tumor histological differentiation, but not with the patients' age or histology type.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>VEGF<\/jats:styled-content>\u2010D<\/jats:styled-content> may play an important role in the process of lymphatic metastasis of epithelial ovarian cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajco.12086","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T01:56:03Z","timestamp":1375754163000},"source":"Crossref","is-referenced-by-count":1,"title":["Expression of VEGF<\/scp>\u2010D<\/scp> in epithelial ovarian cancer and its relationship to lymphatic metastasis"],"prefix":"10.1111","volume":"12","author":[{"given":"Lixia","family":"He","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyong","family":"He","sequence":"additional","affiliation":[{"name":"Physical Examination Center West China Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11675"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.17.4762"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/84635"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/7FXVRMXF58PVRJUH"},{"key":"e_1_2_6_6_1","first-page":"2179","article-title":"Relation of vascular endothelial growth factor\u2010D expression to microvessel density, microlymphatic vessel density, and lymph\u2010node metastasis of lung adenocarcinoma","volume":"88","author":"Cong B","year":"2008","journal-title":"Zhonghua Yi Xue Za Zhi"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000101855"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.00.3467"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600701"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0379"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3816\/CBC.2003.n.041"},{"key":"e_1_2_6_12_1","first-page":"229","article-title":"Expression analysis of vascular endothelial growth factors and their relationships to lymph node metastasis in human colorectal cancer","volume":"22","author":"Kawakami M","year":"2003","journal-title":"J Exp Clin Cancer Res"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010801)92:3<556::AID-CNCR1355>3.0.CO;2-Q"},{"key":"e_1_2_6_14_1","first-page":"2431","article-title":"Expression of vascular endothelial growth factors A, B, C, and D and their relationships to lymph node status in lung adenocarcinoma","volume":"6","author":"Niki T","year":"2000","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.4.789"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(02)00189-5"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc863"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-669"},{"key":"e_1_2_6_19_1","first-page":"203","article-title":"Regulation of angiogenesis via vascular endothelial growth factor receptors","volume":"60","author":"Veikkola T","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203855"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.9.6584"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2008.01.011"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04480"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0801695"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2605"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.21228"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2008.23.4.592"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2008.12.006"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.4156"},{"key":"e_1_2_6_30_1","first-page":"669","article-title":"Vascular endothelial growth factor\u2010D induces lymphangiogenesis and lymphatic metastasis in models of ductal pancreatic cancer","volume":"27","author":"Von Marschall Z","year":"2005","journal-title":"Int J Oncol"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-28-98"},{"key":"e_1_2_6_32_1","first-page":"240","article-title":"Relationship between clinicopathologic characteristics and expression of VEGF\u2010C and VEGF\u2010D in esophageal squamous cancer","volume":"40","author":"Jiang M","year":"2009","journal-title":"Sichuan Da Xue Xue Bao Yi Xue Ban"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20845"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo_00000193"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1996.0249"}],"container-title":["Asia-Pacific Journal of Clinical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fajco.12086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fajco.12086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajco.12086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T11:47:02Z","timestamp":1694346422000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ajco.12086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,5]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/ajco.12086"],"URL":"https:\/\/doi.org\/10.1111\/ajco.12086","archive":["Portico"],"relation":{},"ISSN":["1743-7555","1743-7563"],"issn-type":[{"value":"1743-7555","type":"print"},{"value":"1743-7563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,5]]}}}